Intracellular oxidation of pyridine nucleotides induces a conformational change in NQO1. David Siegel Donna D. Dehn Samantha S. Bokatzian Kevin Quinn Donald S. Backos Andrea Di Francesco Michel Bernier Nichole Reisdorph Rafael de Cabo David Ross 10.1371/journal.pone.0190717.g004 https://plos.figshare.com/articles/figure/Intracellular_oxidation_of_pyridine_nucleotides_induces_a_conformational_change_in_NQO1_/5755239 <p>(A) 16HBE cells were treated with DMSO or β-lapachone (10μM) for 2 h after which NQO1 was immunoprecipitated using anti-NQO1 antibodies. (B) Human cell lines (16HBE, ARPE19, TrHBMEC) were treated with β-lapachone (10μM) for the indicated times after which NQO1 was immunoprecipitated using antibodies that recognize the C-terminal domain of NQO1. (C) Intracellular levels of NADH and NAD<sup>+</sup> were measured by mass spectrometry in 16HBE cells treated with β-lapachone (10μM) for 2 h in the absence and presence of the PARP inhibitor olaparib (1μM). Results are the mean ± standard deviation, n = 3. (D) Immunoprecipitation of NQO1 from 16HBE cells treated with β-lapachone (10μM) in the absence and presence of olaparib (1μM) for 2 h. Reaction conditions are described in <i>Materials and methods</i>.</p> 2018-01-03 18:40:37 NAD NQO 1 NQO 1 deacetylase Sirt 2 NQO 1 structure Sirt 2-mediated deacetylation NQO 1 co-localize conditions NQO 1 NQO 1 pyridine nucleotides results centriole duplication cycle NQO 1 acetyl α- tubulin β- lapachone FAD