Siegel, David Dehn, Donna D. Bokatzian, Samantha S. Quinn, Kevin Backos, Donald S. Di Francesco, Andrea Bernier, Michel Reisdorph, Nichole de Cabo, Rafael Ross, David Reduced pyridine nucleotides induce a conformational change in NQO1 preventing antibodies from binding to the C-terminus. <p>(A) Immunoblot analysis of rhNQO1 following incubation with trypsin in the absence and presence of NADH. (B, C) Immunoprecipitation of rhNQO1 by antibodies that target the C-terminal domain in the absence and presence of NADH or NADPH. (D) Immunoprecipitation of rhNQO1 by antibodies that target the C-terminal domain in the absence and presence of reduced nicotinamide analogs nicotinamide riboside (NRH) and benzyl dihydronicotinamide (BN). (E) Immunoprecipitation of rhNQO1 by antibodies that target the C-terminal domain following the oxidation of NADH by β-lapachone. (F) Immunoprecipitation of NQO1*1 and NQO1*2 proteins by antibodies that target the C-terminal domain in the absence and presence of NADH. Reaction conditions for immunoprecipitation studies are described in <i>Materials and methods</i>.</p> NAD;NQO 1 NQO 1;deacetylase Sirt 2;NQO 1 structure;Sirt 2-mediated deacetylation;NQO 1 co-localize;conditions NQO 1;NQO 1;pyridine nucleotides results;centriole duplication cycle NQO 1;acetyl α- tubulin;β- lapachone;FAD 2018-01-03
    https://plos.figshare.com/articles/figure/Reduced_pyridine_nucleotides_induce_a_conformational_change_in_NQO1_preventing_antibodies_from_binding_to_the_C-terminus_/5755230
10.1371/journal.pone.0190717.g002