The specific lytic activity of MCF-7 cancer stem-like cell antigens. Yoo Jin Choi Seong-Joon Park You-Soo Park Hee Sung Park Kwang Mo Yang Kyu Heo 10.1371/journal.pone.0190638.g001 https://plos.figshare.com/articles/figure/The_specific_lytic_activity_of_MCF-7_cancer_stem-like_cell_antigens_/5755020 <p>(A) Identification of populations of MCF-7 cells by flow cytometry analysis of CD44 and CD24 expression. (B) Western blot showing CD44 and ALDH protein expression in CD44<sup>+</sup>, CD44<sup>-</sup> and CD44<sup>+</sup>CD24<sup>-</sup> MCF7- cells. (C) Targeted killing of CD44<sup>+</sup> MCF-7 cells by tumor-associated antigen-pulsed, DC-induced CTLs. The data are expressed as the mean±SD, and significant differences between the treated groups were detected using ANOVA followed by the Dunnett’s test. <sup>#</sup> <i>p</i><0.05 compared with the DC-CTLs. * <i>p</i><0.05 compared with the TL-CTLs.</p> 2018-01-03 18:38:33 peptide-T 2 binding assay EpCAM peptides anti-tumor immunity HLA-A 2 molecules antigen CTL CSC marker MHC cytotoxic T lymphocytes HCC CD 44 T cell stimulation breast cancer EpCAM peptide-primed dendritic cell vaccination TAA hepatocellular carcinoma cells Cancer stem-like cells DC