10.1371/journal.pone.0190638.g001
Yoo Jin Choi
Yoo Jin
Choi
Seong-Joon Park
Seong-Joon
Park
You-Soo Park
You-Soo
Park
Hee Sung Park
Hee Sung
Park
Kwang Mo Yang
Kwang Mo
Yang
Kyu Heo
Kyu
Heo
The specific lytic activity of MCF-7 cancer stem-like cell antigens.
Public Library of Science
2018
peptide-T 2 binding assay
EpCAM peptides
anti-tumor immunity
HLA-A 2 molecules
antigen
CTL
CSC marker
MHC
cytotoxic T lymphocytes
HCC
CD 44
T cell stimulation
breast cancer
EpCAM peptide-primed dendritic cell vaccination
TAA
hepatocellular carcinoma cells Cancer stem-like cells
DC
2018-01-03 18:38:33
Figure
https://plos.figshare.com/articles/figure/The_specific_lytic_activity_of_MCF-7_cancer_stem-like_cell_antigens_/5755020
<p>(A) Identification of populations of MCF-7 cells by flow cytometry analysis of CD44 and CD24 expression. (B) Western blot showing CD44 and ALDH protein expression in CD44<sup>+</sup>, CD44<sup>-</sup> and CD44<sup>+</sup>CD24<sup>-</sup> MCF7- cells. (C) Targeted killing of CD44<sup>+</sup> MCF-7 cells by tumor-associated antigen-pulsed, DC-induced CTLs. The data are expressed as the mean±SD, and significant differences between the treated groups were detected using ANOVA followed by the Dunnett’s test. <sup>#</sup> <i>p</i><0.05 compared with the DC-CTLs. * <i>p</i><0.05 compared with the TL-CTLs.</p>