10.6084/m9.figshare.5746593.v1
Yu Zhang
Yu
Zhang
Tao Zhou
Tao
Zhou
Li Luo
Li
Luo
Zheng Cui
Zheng
Cui
Na Wang
Na
Wang
Yamin Shu
Yamin
Shu
Kai-Ping Wang
Kai-Ping
Wang
Pharmacokinetics, biodistribution and receptor mediated endocytosis of a natural <i>Angelica sinensis</i> polysaccharide
Taylor & Francis Group
2018
Angelica sinensis
natural polysaccharide
pharmacokinetics
biodistribution
asialoglycoprotein receptor
in vivo imaging
2018-01-02 07:11:04
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Pharmacokinetics_biodistribution_and_receptor_mediated_endocytosis_of_a_natural_i_Angelica_sinensis_i_polysaccharide/5746593
<p>The interest in developing new drug carriers for delivery to the liver using natural polysaccharides with a high galactose content has necessitated the study of the pharmacokinetics and tissue distribution of these polysaccharides. In this paper, a new method was established for the microanalysis of <i>Angelica sinensis</i> polysaccharide (ASP) in biosamples. Fluorescein-labelled ASP (FA) was rapidly eliminated from the bloodstream and distributed to the liver with high specificity following intravenous injection. The analysis of the hepatocellular localization demonstrated that FA was predominantly endocytosed by the parenchymal cells, an observation consistent with the results obtained from microscopy studies. Additionally, FA showed a high affinity for asialoglycoprotein receptor-rich cells, while minimal binding of FA to asialoglycoprotein receptor-poor cells was observed. Moreover, the absorption of FA was markedly inhibited by the co-administration of neogalactosylalbumin (NGA) both <i>in vivo</i> and <i>in vitro</i>. To allow for the visualization of the systemic circulation of ASP, <sup>99m</sup>Tc-DTPA-ASP was synthesized and <i>in vivo</i> imaging was performed with single photon emission computed tomography (SPECT). It also showed a high aggregation of <sup>99m</sup>Tc-DTPA-ASP in liver. These results suggest that the distribution of ASP to the liver occurs via asialoglycoprotein receptor (ASGPR) mediated endocytosis and ASP could potentially be applied as a new carrier for delivering drugs to the liver.</p>