10.6084/m9.figshare.5739567.v1 Hamdy K. Assaf Hamdy K. Assaf Alaa M. Nafady Alaa M. Nafady Ahmed E. Allam Ahmed E. Allam Ashraf N. E. Hamed Ashraf N. E. Hamed Mohamed S. Kamel Mohamed S. Kamel Kuniyoshi Shimizu Kuniyoshi Shimizu Forsskamide, a new ceramide from aerial parts of <i>Forsskaolea tenacissima</i> Linn. Taylor & Francis Group 2017 Forsskaolea tenacissima Urticaceae Forsskamide ceramide and cytotoxicity 2017-12-28 05:26:25 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Forsskamide_a_new_ceramide_from_aerial_parts_of_i_Forsskaolea_tenacissima_i_Linn_/5739567 <p>Although the various folk medicine uses and the biological activity of <i>Forsskaolea tenacissima</i> L., few chemical constituents of this plant have been reported, this provoked us to make our study. Forsskamide, a new ceramide was isolated from aerial parts of <i>F. tenacissima</i> L. (Urticaceae). The chemical structure was established by different spectroscopic methods (<sup>1</sup>H, <sup>13</sup>C-NMR, HMBC, HSQC, ROESY, FAB-MS and HR-FAB-MS). Forsskamide showed a moderate cytotoxic activity by (MTT) method against human colorectal carcinoma cell line (HCT-116) with IC<sub>50</sub> 33.25 μM in comparison with 5-fluorouracil IC<sub>50</sub> 26.42 μM. While, it did not show any activity against human hepatocarcinaoma cell line (HepG-2).</p>