TY - DATA T1 - HPLC-DAD DETERMINATION OF BERBERINE, CHELERYTHRINE AND SANGUINARINE IN THE MEXICAN PRICKLY POPPY (Argemone mexicana L. PAPAVERACEAE), A MEDICINAL PLANT PY - 2017/12/27 AU - Jorge F. Xool-Tamayo AU - Manlio Graniel-Sabido AU - Gumersindo Mirón-López AU - Gonzalo J. Mena-Rejón AU - Miriam Monforte-González AU - Felipe Vázquez Flota UR - https://scielo.figshare.com/articles/dataset/HPLC-DAD_DETERMINATION_OF_BERBERINE_CHELERYTHRINE_AND_SANGUINARINE_IN_THE_MEXICAN_PRICKLY_POPPY_Argemone_mexicana_L_PAPAVERACEAE_A_MEDICINAL_PLANT/5734662 DO - 10.6084/m9.figshare.5734662.v1 L4 - https://ndownloader.figshare.com/files/10094076 L4 - https://ndownloader.figshare.com/files/10094079 L4 - https://ndownloader.figshare.com/files/10094082 L4 - https://ndownloader.figshare.com/files/10094085 L4 - https://ndownloader.figshare.com/files/10094088 L4 - https://ndownloader.figshare.com/files/10094091 L4 - https://ndownloader.figshare.com/files/10094094 L4 - https://ndownloader.figshare.com/files/10094097 L4 - https://ndownloader.figshare.com/files/10094100 KW - Argemone mexicana KW - berberine KW - chelerythrine KW - prickly poppy KW - sanguinarine N2 - A sensitive, simple, rapid and reliable HPLC-DAD method for the analysis of the benzylisoquinoline alkaloids (BIA's) content in Argemone mexicana (Papaveraceae) is presented. This method allows the simultaneous separation and quantitation of berberine (Bn), chelerythrine (C) and sanguinarine (S) in extracts from A. mexicana tissues, reducing time of analysis in comparison to previous reports. Alkaloids were separated on a C18 Hypersil Gold column using an acetonitrile gradient (20 to 70%) in 1% acetic acid in water. Alkaloids were identified based on retention times and UV spectra and quantified at 254 nm. Linearity between 0.5-20 µg mL-1 was observed for Bn, C and S, with limits of detection (LOD) and quantitation (LOQ) of 0.11 and 0.33 for Bn, 0.10 and 0.30 for C and 0.05 and 0.15 for S, respectively. Maximal intra- and inter-day variation values were < 0.49% in all cases, with alkaloids' recoveries higher than 95%. System suitability tests (SST), including resolution (Rs), retention factor (K'), selectivity (α), tailing factor and number of theoretical plates were performed according to the United States Pharmacopeia (USP), fulfilling recommended values. The method proved to be efficient and reproducible when analyzing different tissues of field-collected A. mexicana plants. ER -