10.6084/m9.figshare.5734245.v1
Michele Trombin-Souza
Michele
Trombin-Souza
Camila Ribeiro de Souza Grzybowski
Camila Ribeiro de Souza
Grzybowski
Yohana de Oliveira-Cauduro
Yohana de
Oliveira-Cauduro
Elisa Serra Negra Vieira
Elisa Serra Negra
Vieira
Maristela Panobianco
Maristela
Panobianco
Osmotic stress on genetically transformed tobacco plant seeds
SciELO journals
2017
Nicotiana tabacum L
proline
saline stress
water deficit
physiological quality
2017-12-27 02:40:53
Dataset
https://scielo.figshare.com/articles/dataset/Osmotic_stress_on_genetically_transformed_tobacco_plant_seeds/5734245
<div><p>Abstract: Salinity and water deficit limit the productivity of several crops; thus, studies related to the genetic transformation of seeds in a model plant, such as tobacco, can be an alternative to minimize negative impacts caused by environmental conditions. The purpose of this work was to evaluate the tolerance to osmotic stress of seeds from genetically transformed tobacco plants, with the introduction of the proline-synthesizer gene (p5csf129a), under salinity and water deficit conditions. To do so, five events with differences in proline content were selected, ranging from 0.70 to 10.47 µmoles.g-1 of fresh mass. The used saline concentrations were: zero (distilled water); 50; 100; 150 and 200 mmol.L-1 of NaCl, whereas for the water deficit, simulated with PEG 6000, the following osmotic potentials were used: zero (distilled water); -0.2; -0.4; -0.6 and -0.8 MPa. Each tested treatment was evaluated through germination, first germination count and germination speed index tests. It is possible to conclude that seeds from genetically transformed tobacco plants with overexpression of the gene p5csf129a, a proline synthesizer, are more tolerant to osmotic stresses. Tabacco seeds with a proline content of 10.47 µmol.g-1 showed a better perfomance, revealing higher physiological potential.</p></div>