TY - DATA T1 - SlWRKY45, nematode-responsive tomato WRKY gene, enhances susceptibility to the root knot nematode; M. javanica infection PY - 2017/12/22 AU - Bharathiraja Chinnapandi AU - Patricia Bucki AU - Sigal Braun Miyara UR - https://tandf.figshare.com/articles/dataset/_i_SlWRKY45_i_nematode-responsive_tomato_i_WRKY_i_gene_enhances_susceptibility_to_the_root_knot_nematode_i_M_javanica_i_infection/5729457 DO - 10.6084/m9.figshare.5729457.v1 L4 - https://ndownloader.figshare.com/files/10076811 L4 - https://ndownloader.figshare.com/files/10076814 L4 - https://ndownloader.figshare.com/files/10076817 L4 - https://ndownloader.figshare.com/files/10076820 KW - Basal resistance KW - innate immunity KW - Meloidogyne javanica KW - plant defense signaling KW - phytohormones KW - root susceptibility KW - WRKY transcription factor N2 - The fluctuation of tomato's WRKY defense regulators during infection by the root knot nematode Meloidogyne javanica was analyzed: and the spatial and temporal expression of SlWRKY45 was studied in depth with regard to its response to nematode infection, phytohormones, and wounding. Expression of WRKY45 increased substantially within 5 d upon infection and continued through feeding-site development and gall maturation. Histological analysis of nematode feeding sites indicated that WRKY45 was highly expressed within the feeding cells and associated vascular parenchyma cells. Responses of SlWRKY45 promoters to several phytohormones showed that WRKY45 was highly induced by specific phytohormones, including cytokinin, auxin, and the defense-signaling molecule salicylic acid (SA), but not by the jasmonates. Overexpressing tomato lines were generated, and infection tests showed that, significantly, roots over-expressing SlWRKY45 contained substantially increased number of females, indicating that WRKY45 overexpression supported faster nematode development. qRT-PCR tests have shown roots overexpressing WRKY45 suppressed the jasmonic acid and salicylic acid marker genes, proteinase inhibitor (PI), and pathogenesis related protein (PR1), respectively, and also the cytokinin response factors CRF1 and CRF6. Overall, this study indicated SlWRKY45 to be a potential transcription factor whose manipulation by the invading nematode might be critical for coordination of hormone signals supporting favorable condition for nematode development in root tissue. ER -