10.1371/journal.pone.0189586.g012 Maëlle Lorvellec Maëlle Lorvellec Federico Scottoni Federico Scottoni Claire Crowley Claire Crowley Rebeca Fiadeiro Rebeca Fiadeiro Panagiotis Maghsoudlou Panagiotis Maghsoudlou Alessandro Filippo Pellegata Alessandro Filippo Pellegata Francesca Mazzacuva Francesca Mazzacuva Asllan Gjinovci Asllan Gjinovci Anne-Marie Lyne Anne-Marie Lyne Justine Zulini Justine Zulini Daniel Little Daniel Little Olukunbi Mosaku Olukunbi Mosaku Deirdre Kelly Deirdre Kelly Paolo De Coppi Paolo De Coppi Paul Gissen Paul Gissen Lipid storage by hiPSC- or hESC-derived hepatocytes in 2D or in 3D on day 13 of differentiation. Public Library of Science 2017 hepatocyte marker albumin 2 D cultures hepatocyte marker alpha-fetoprotein 2 D conditions hepatocyte-like cells Liver transplantation regenerative medicine applications Pluripotent Stem Cells cell hepatocyte differentiation 3 D culture organ regeneration therapies optimised 3 D protocols blood vessel network mouse decellularised liver scaffold 3 D donor organ shortage limits hepatocyte differentiation hiPSC 2017-12-20 18:38:46 Figure https://plos.figshare.com/articles/figure/Lipid_storage_by_hiPSC-_or_hESC-derived_hepatocytes_in_2D_or_in_3D_on_day_13_of_differentiation_/5724613 <p>(a-b) Lipids detected by ORO (droplets, red-orange). Nuclei stained with hematoxylin (purple). Substantially higher percentage of cells are ORO positive in 3D (b) than in 2D (a) on day 13. (c) Boxplots of percentages of positive cells for ORO for each group. The bottom and top of the box are the first and third quartiles, and the band inside the box is the median. The ends of the whiskers represent the minimum and maximum. Individual data points represented by ○ and ▲ are mild and extreme outliers, respectively. Statistical difference level is indicated by ** for p<0.01 according to the results of the Dunn-Bonferroni posthoc tests performed with the Kruskal-Wallis test (α = 0.05).</p>