10.1371/journal.pone.0189586.g012
Maëlle Lorvellec
Maëlle
Lorvellec
Federico Scottoni
Federico
Scottoni
Claire Crowley
Claire
Crowley
Rebeca Fiadeiro
Rebeca
Fiadeiro
Panagiotis Maghsoudlou
Panagiotis
Maghsoudlou
Alessandro Filippo Pellegata
Alessandro Filippo
Pellegata
Francesca Mazzacuva
Francesca
Mazzacuva
Asllan Gjinovci
Asllan
Gjinovci
Anne-Marie Lyne
Anne-Marie
Lyne
Justine Zulini
Justine
Zulini
Daniel Little
Daniel
Little
Olukunbi Mosaku
Olukunbi
Mosaku
Deirdre Kelly
Deirdre
Kelly
Paolo De Coppi
Paolo
De Coppi
Paul Gissen
Paul
Gissen
Lipid storage by hiPSC- or hESC-derived hepatocytes in 2D or in 3D on day 13 of differentiation.
Public Library of Science
2017
hepatocyte marker albumin
2 D cultures
hepatocyte marker alpha-fetoprotein
2 D conditions
hepatocyte-like cells Liver transplantation
regenerative medicine applications
Pluripotent Stem Cells
cell hepatocyte differentiation
3 D culture
organ regeneration therapies
optimised 3 D protocols
blood vessel network
mouse decellularised liver scaffold
3 D
donor organ shortage limits
hepatocyte differentiation
hiPSC
2017-12-20 18:38:46
Figure
https://plos.figshare.com/articles/figure/Lipid_storage_by_hiPSC-_or_hESC-derived_hepatocytes_in_2D_or_in_3D_on_day_13_of_differentiation_/5724613
<p>(a-b) Lipids detected by ORO (droplets, red-orange). Nuclei stained with hematoxylin (purple). Substantially higher percentage of cells are ORO positive in 3D (b) than in 2D (a) on day 13. (c) Boxplots of percentages of positive cells for ORO for each group. The bottom and top of the box are the first and third quartiles, and the band inside the box is the median. The ends of the whiskers represent the minimum and maximum. Individual data points represented by ○ and ▲ are mild and extreme outliers, respectively. Statistical difference level is indicated by ** for p<0.01 according to the results of the Dunn-Bonferroni posthoc tests performed with the Kruskal-Wallis test (α = 0.05).</p>