%0 Figure %A Lorvellec, Maƫlle %A Scottoni, Federico %A Crowley, Claire %A Fiadeiro, Rebeca %A Maghsoudlou, Panagiotis %A Pellegata, Alessandro Filippo %A Mazzacuva, Francesca %A Gjinovci, Asllan %A Lyne, Anne-Marie %A Zulini, Justine %A Little, Daniel %A Mosaku, Olukunbi %A Kelly, Deirdre %A De Coppi, Paolo %A Gissen, Paul %D 2017 %T ASGPR expression by hiPSC- or hESC-derived hepatocytes in 2D or 3D on day 13 of differentiation. %U https://plos.figshare.com/articles/figure/ASGPR_expression_by_hiPSC-_or_hESC-derived_hepatocytes_in_2D_or_3D_on_day_13_of_differentiation_/5724601 %R 10.1371/journal.pone.0189586.g011 %2 https://ndownloader.figshare.com/files/10066687 %K hepatocyte marker albumin %K 2 D cultures %K hepatocyte marker alpha-fetoprotein %K 2 D conditions %K hepatocyte-like cells Liver transplantation %K regenerative medicine applications %K Pluripotent Stem Cells %K cell hepatocyte differentiation %K 3 D culture %K organ regeneration therapies %K optimised 3 D protocols %K blood vessel network %K mouse decellularised liver scaffold %K 3 D %K donor organ shortage limits %K hepatocyte differentiation %K hiPSC %X

(a-b) Immunostaining of hepatocyte sinusoidal membrane protein ASGPR in 2D (a) and 3D (b) cultured cells. Nuclei stained with DAPI. The higher magnification images of merged channels in the bottom panel correspond to the delineated lower magnification images in the panel above. Images of 3D cultured cells demonstrate membrane localisation of ASGPR.

%I PLOS ONE