TY - DATA T1 - The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline PY - 2017/12/20 AU - Teresinha Regina Ribeiro de Oliveira AU - Geraldo Ferreira de Oliveira AU - Ricardo Santos Simões AU - Suellen Maurim Feitosa AU - Eduardo Hiroshi Tikazawa AU - Hugo Pequeno Monteiro AU - Djalma José Fagundes AU - Murched Omar Taha UR - https://scielo.figshare.com/articles/dataset/The_expression_of_endothelial_and_inducible_nitric_oxide_synthase_and_apoptosis_in_intestinal_ischemia_and_reperfusion_injury_under_the_action_of_ischemic_preconditioning_and_pentoxifylline/5719444 DO - 10.6084/m9.figshare.5719444.v1 L4 - https://ndownloader.figshare.com/files/10045258 L4 - https://ndownloader.figshare.com/files/10045264 L4 - https://ndownloader.figshare.com/files/10045273 L4 - https://ndownloader.figshare.com/files/10045279 L4 - https://ndownloader.figshare.com/files/10045285 L4 - https://ndownloader.figshare.com/files/10045288 KW - Ischemic Preconditioning KW - Pentoxifylline KW - Ischemia KW - Reperfusion Injury KW - Nitric Oxide Synthase KW - Apoptosis KW - Rats. N2 - Abstract Purpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes). Results: The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P<0.05) compared to IR+SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P>0.05). The NOS-2 expression (qRTPCR) in the IR-PTX group (P<0.05) was higher than the values for the IPC+IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P<0.05), the IR-SS (P<0.05) and the IR-PTX (P<0.05) groups. Conclusions: The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect. ER -