TY - DATA T1 - Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs PY - 2017/12/20 AU - Eduardo Gustavo Pires de Arruda AU - Alexandre Mendonça Munhoz AU - Walter Matsumoto AU - Thiago Ueda AU - Renata de Almeida Coudry AU - Rolf Gemperli UR - https://scielo.figshare.com/articles/dataset/Qualitative_analysis_of_the_viability_of_autogenous_fat_grafts_grafted_in_different_environments_of_interstitial_pressure_Preliminary_results_and_description_of_a_new_experimental_model_in_mini-pigs/5719228 DO - 10.6084/m9.figshare.5719228.v1 L4 - https://ndownloader.figshare.com/files/10043884 L4 - https://ndownloader.figshare.com/files/10043893 L4 - https://ndownloader.figshare.com/files/10043911 L4 - https://ndownloader.figshare.com/files/10043920 L4 - https://ndownloader.figshare.com/files/10043938 L4 - https://ndownloader.figshare.com/files/10043950 L4 - https://ndownloader.figshare.com/files/10043962 L4 - https://ndownloader.figshare.com/files/10043977 L4 - https://ndownloader.figshare.com/files/10044007 L4 - https://ndownloader.figshare.com/files/10044022 L4 - https://ndownloader.figshare.com/files/10044037 L4 - https://ndownloader.figshare.com/files/10044064 L4 - https://ndownloader.figshare.com/files/10044073 KW - Mammaplasty KW - Models, Animal KW - Swine. N2 - Abstract Purpose: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. Methods: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure “under tension”. A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. Results: The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. Conclusion: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response. ER -