TY - DATA T1 - Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) PY - 2017/12/13 AU - Betania Barros Cota AU - Luiza Guimarães Tunes AU - Daniela Nabak Bueno Maia AU - Jonas Pereira Ramos AU - Djalma Menezes de Oliveira AU - Markus Kohlhoff AU - Tânia Maria de Almeida Alves AU - Elaine Maria Souza-Fagundes AU - Fernanda Fraga Campos AU - Carlos Leomar Zani UR - https://scielo.figshare.com/articles/dataset/Leishmanicidal_compounds_of_Nectria_pseudotrichia_an_endophytic_fungus_isolated_from_the_plant_Caesalpinia_echinata_Brazilwood_/5695807 DO - 10.6084/m9.figshare.5695807.v1 L4 - https://ndownloader.figshare.com/files/9975247 L4 - https://ndownloader.figshare.com/files/9975280 L4 - https://ndownloader.figshare.com/files/9975292 L4 - https://ndownloader.figshare.com/files/9975298 L4 - https://ndownloader.figshare.com/files/9975307 L4 - https://ndownloader.figshare.com/files/9975316 L4 - https://ndownloader.figshare.com/files/9975322 L4 - https://ndownloader.figshare.com/files/9975328 KW - fungal metabolites KW - natural products KW - Leishmania braziliensis KW - antileishmanial KW - neglected disease N2 - BACKGROUND In a screen of extracts from plants and fungi to detect antileishmanial activity, we found that the ethyl acetate extract of the fungus Nectria pseudotrichia, isolated from the tree Caesalpinia echinata (Brazilwood), is a promising source of bioactive compounds. OBJECTIVES The aims of this study were to isolate and determine the chemical structures of the compounds responsible for the antileishmanial activity of the organic extract from N. pseudotrichia. METHODS Compounds were isolated by chromatographic fractionation using semi-preparative high-performance liquid chromatography, and their chemical structures were determined by analytical and spectral data and by comparison with published data. The antileishmanial activity of the isolated compounds was evaluated in intracellular amastigote forms of Leishmania (Viannia) braziliensis expressing firefly luciferase as reporter gene, and cytotoxicity was determined in Vero and THP-1 mammalian cell lines by MTT assay. FINDINGS Fractionation of the extract yielded seven compounds: 10-acetyl trichoderonic acid A (1), 6′-acetoxy-piliformic acid (2), 5′,6′-dehydropiliformic acid (3), piliformic acid (4), hydroheptelidic acid (5), xylaric acid D (6), and cytochalasin D (7). Compounds 1, 2 and 3 are reported here for the first time. Compounds 1, 2, and 5 were more active, with IC50 values of 21.4, 28.3, and 24.8 µM, respectively, and showed low toxicity to Vero and THP-1 cells. MAIN CONCLUSIONS N. pseudotrichia produces secondary metabolites that are more toxic to intracellular amastigote forms of L. (V.) braziliensis than to mammalian cells. ER -