TY - DATA T1 - Structure determination and quantification of a new flavone glycoside with anti-acetylcholinesterase activity from the herbs of Elsholtzia ciliata PY - 2017/12/11 AU - Agung Nugroho AU - Jong-Hyuk Park AU - Jae Sue Choi AU - Kyoung-Sik Park AU - Joon-Pyo Hong AU - Hee-Juhn Park UR - https://tandf.figshare.com/articles/journal_contribution/Structure_determination_and_quantification_of_a_new_flavone_glycoside_with_anti-acetylcholinesterase_activity_from_the_herbs_of_i_Elsholtzia_ciliata_i_/5687872 DO - 10.6084/m9.figshare.5687872.v1 L4 - https://ndownloader.figshare.com/files/9957913 KW - Elsholtzia ciliata KW - Labiatae KW - acacetin triglycoside KW - acacetin KW - NMR KW - HPLC N2 - Three acacetin triglycosides (compounds 1, 2 and 3) were isolated from the herbs of Elsholtzia ciliata (Labiatae). The structure were identified as 7-O-β-D-glucopyranosyl-(1 → 2)[α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside (compound 1), 7-O-(6-O-acetyl)-β-D-glucopyranosyl-(1 → 2)[α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside (compound 2) and 7-O-(6-O-acetyl)-β-D-glucopyranosyl-(1 → 2)[(4-O-acetyl)-α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside (compound 3) of acacetin. The structures of these compounds were determined on the basis of 2D-NMR spectroscopic data. Compound 3 has not been isolated from a natural source. In addition, the three compounds were quantitatively analysed by HPLC. Acetylcholinesterase (AChE) inhibition activity was assayed to find anti-Alzheimer’s activity, since this enzyme increases the concentration of acetylcholine (ACh), a neurotransmitter, responsible for brain’s memory. Acacetin, the aglycone of the three compounds, exhibited a potent anti-cholinesterase activity (IC50, 50.33 ± 0.87), though its glycosides (1, 2 and 3) were less active. HPLC analysis demonstrated that the three compounds were contained in the MeOH extract in the order of compounds 2 (12.63 mg/g extract) > 3 (3.10 mg/g) > 1 (2.92 mg/g). ER -