10.6084/m9.figshare.5682649.v1
Arandeep Dhanda
Arandeep
Dhanda
A Wayne Vogl
A Wayne
Vogl
Sharifah E Albraiki
Sharifah E
Albraiki
Carol A Otey
Carol
A Otey
Moriah R Beck
Moriah R
Beck
Julian A Guttman
Julian A
Guttman
Supplemental Figure 4. Actin-Rich Cytoplasmic Clumps Are Generated in PalladinFPAA-Expressing Cells.
figshare
2017
Listeria monocytogenes
Cell Biology
Microbiology
2017-12-07 20:22:57
Figure
https://figshare.com/articles/figure/Supplemental_Figure_4_Actin-Rich_Cytoplasmic_Clumps_Are_Generated_in_PalladinFPAA-Expressing_Cells_/5682649
<p>HeLa cells were transfected with various GFP-tagged palladin
DNA constructs (indicated on left), fixed and stained with DAPI (blue) to
visualize DNA and Alexa594-phalloidin to visualize actin and simultaneously
observe for general cytoskeletal alterations. </p>
<p>(A) Palladin displays robust punctate staining at actin stress
fibers in GFP-palladin transfected cells. In GFP-palladinFPAA cells, actin
stress fibers are generally absent from within the central body of the cell and
abnormal actin-rich cytoplasmic clumps (open arrowheads) and bar-like
structures (solid arrowheads) are formed. Palladin retains some punctate
staining at residual stress fibers within the cell. The actin cytoskeleton is
disorganized and palladin is redistributed to the nucleus in
GFP-palladinK15/18/51A transfected cells. Scale bar, 5 µm (also applies to B).</p>
<p>(B) Endogenous VASP (red) is redistributed to actin-rich
cytoplasmic clumps and bar-like structures formed in GFP-palladinFPAA
transfected cells.</p>