<i>w</i>MelCS provides superior blocking of DENV-3 genome replication in a mosquito injection challenge model. Johanna E. Fraser Jyotika Taneja De Bruyne Iñaki Iturbe-Ormaetxe Justin Stepnell Rhiannon L. Burns Heather A. Flores Scott L. O’Neill 10.1371/journal.ppat.1006751.g005 https://plos.figshare.com/articles/figure/_i_w_i_MelCS_provides_superior_blocking_of_DENV-3_genome_replication_in_a_mosquito_injection_challenge_model_/5682496 <p>(A) DENV-3 was injected into the thorax of 6 or 7-day old female mosquitoes at 2.5 x10<sup>6</sup> TCID<sub>50</sub>/ml (undiluted) or 10, 100 or 1000-fold dilutions thereof. RNA was extracted from whole mosquito bodies 7-days post infection and virus replication was quantified by qRT-PCR. Data are the mean number of genome copies per mosquito ± SEM. Number of DENV-3 positive mosquitoes/total <i>n</i> are indicated above each bar. ** p < 0.01, ****p<0.0001, Mann-Whitney test. (B) The mean DENV genome copies and SEM from (A) are replotted as a function of virus concentration injected. Significant differences in the mean RNA copies of <i>w</i>Mel and <i>w</i>MelCS lines are indicated by ** (p<0.01). Significant differences in the mean RNA copies of <i>w</i>Mel.Tet and <i>w</i>MelCS.Tet lines are indicated by <b>‡</b> (p<0.05), Mann-Whitney test. Injection dilutions were performed as independent experiments, with the data combined to produce the final data series.</p> 2017-12-07 18:59:41 field-relevant fitness tests Mel vector competence phenotypes Wolbachia pipientis RNA virus alternative Wolbachia strain Wolbachia strains future field testing MelCS transinfected Aedes aegypti mosquitoes mosquito vector Aedes aegypti