RABV-G mRNA vaccine stored at temperatures up to 70°C retains immunogenicity and protection for up to three months.
Lothar Stitz
Annette Vogel
Margit Schnee
Daniel Voss
Susanne Rauch
Thorsten Mutzke
Thomas Ketterer
Thomas Kramps
Benjamin Petsch
10.1371/journal.pntd.0006108.g002
https://plos.figshare.com/articles/figure/RABV-G_mRNA_vaccine_stored_at_temperatures_up_to_70_C_retains_immunogenicity_and_protection_for_up_to_three_months_/5682493
<p>mRNA vaccine or licensed vaccines were stored for one month (A-F) or three months (G-I). Storage temperature was 60°C (A-C), 70°C (D-F), or as indicated in the figure (G-I). Vaccine or control injections were done on day 0 and 21. Induction of rabies virus neutralizing antibodies was measured 35 days after first immunization (A, D, G; dashed line indicates the WHO standard titer of 0.5 IU/ml). Mice were challenged with a 40fold MLD<sub>50</sub> of rabies virus CVS-11 on day 47 (B, C, E, F) and day 51 (H-I), respectively. Survival (B, E, H) and body weight (C, F, I) was recorded. For antibody titers, the mean is indicated, for body weight kinetics the mean and standard deviation is given (n = 6 to 8). Significance *p<0.1, **p<0.01, ***p < .001 to HDC or buffer control group in A and D/G, respectively, was calculated using the Mann Whitney test. Groups included in A-F were performed simultaneously; therefore buffer control displayed in D-F is valid for all groups.</p>
2017-12-07 18:59:53
temperature resistance
rabies virus infections
non-replicating mRNA
rabies virus vaccine encoding
virus neutralizing antibodies
20 cycles
rabies virus glycoprotein
rabies infection
rabies virus
mRNA vaccine
thermostable messenger RNA
RABV-G
50.000 fatalities
rabies virus vaccines
cell culture
vaccine transport