RABV-G mRNA vaccine stored at temperatures up to 70°C retains immunogenicity and protection for up to three months. Lothar Stitz Annette Vogel Margit Schnee Daniel Voss Susanne Rauch Thorsten Mutzke Thomas Ketterer Thomas Kramps Benjamin Petsch 10.1371/journal.pntd.0006108.g002 https://plos.figshare.com/articles/figure/RABV-G_mRNA_vaccine_stored_at_temperatures_up_to_70_C_retains_immunogenicity_and_protection_for_up_to_three_months_/5682493 <p>mRNA vaccine or licensed vaccines were stored for one month (A-F) or three months (G-I). Storage temperature was 60°C (A-C), 70°C (D-F), or as indicated in the figure (G-I). Vaccine or control injections were done on day 0 and 21. Induction of rabies virus neutralizing antibodies was measured 35 days after first immunization (A, D, G; dashed line indicates the WHO standard titer of 0.5 IU/ml). Mice were challenged with a 40fold MLD<sub>50</sub> of rabies virus CVS-11 on day 47 (B, C, E, F) and day 51 (H-I), respectively. Survival (B, E, H) and body weight (C, F, I) was recorded. For antibody titers, the mean is indicated, for body weight kinetics the mean and standard deviation is given (n = 6 to 8). Significance *p<0.1, **p<0.01, ***p < .001 to HDC or buffer control group in A and D/G, respectively, was calculated using the Mann Whitney test. Groups included in A-F were performed simultaneously; therefore buffer control displayed in D-F is valid for all groups.</p> 2017-12-07 18:59:53 temperature resistance rabies virus infections non-replicating mRNA rabies virus vaccine encoding virus neutralizing antibodies 20 cycles rabies virus glycoprotein rabies infection rabies virus mRNA vaccine thermostable messenger RNA RABV-G 50.000 fatalities rabies virus vaccines cell culture vaccine transport