TY - DATA T1 - RABV-G mRNA vaccine stored at temperatures up to +40°C retains immunogenicity and protective for up to twelve months. PY - 2017/12/07 AU - Lothar Stitz AU - Annette Vogel AU - Margit Schnee AU - Daniel Voss AU - Susanne Rauch AU - Thorsten Mutzke AU - Thomas Ketterer AU - Thomas Kramps AU - Benjamin Petsch UR - https://plos.figshare.com/articles/figure/RABV-G_mRNA_vaccine_stored_at_temperatures_up_to_40_C_retains_immunogenicity_and_protective_for_up_to_twelve_months_/5682481 DO - 10.1371/journal.pntd.0006108.g001 L4 - https://ndownloader.figshare.com/files/10037989 KW - temperature resistance KW - rabies virus infections KW - non-replicating mRNA KW - rabies virus vaccine encoding KW - virus neutralizing antibodies KW - 20 cycles KW - rabies virus glycoprotein KW - rabies infection KW - rabies virus KW - mRNA vaccine KW - thermostable messenger RNA KW - RABV-G KW - 50.000 fatalities KW - rabies virus vaccines KW - cell culture KW - vaccine transport N2 - (A-B: storage for six months). Vaccine or control injections were done on day 0 and 21. Induction of rabies virus neutralizing antibodies was measured on day 35 in mice vaccinated with RABV-G mRNA stored from -80°C to +40°C or HDC stored at the recommended temperature of 4°C for six months (dashed line in A and C indicates the WHO recommended titer of 0.5 international units per ml [IU/ml]). (C, D: storage for twelve months) Mice were challenged with 40fold MLD50 of rabies virus CVS-11, 71 (A, B) or 63 (C, D) days after the first immunization. Body weight was recorded after immunization using material stored for six (B) or (D) twelve months. For antibody titers, the mean is indicated, for body weight kinetics the mean and standard deviation is given (n = 5). Significance ** p < .001 to buffer control group was calculated using the Mann Whitney test. ER -