L. Richards, Alexsia J. Sollars, Patricia Pitts, Jared D. Stults, Austin M. E. Heldwein, Ekaterina E. Pickard, Gary A. Smith, Gregory Mutation of PRV R2 results in a post-entry microtubule-dependent delay in gene expression. <p><b>(A)</b> Percent of PRV internalized into PK15 epithelial cells prior to extracellular virion inactivation with citrate at indicated times post-infection. Five experimental replicates are shown for each virus, with each representing average values from duplicate infections. <b>(B)</b> Expression of the PRV immediate early gene IE180 was quantified by qRT-PCR and normalized to expression of the host S28 rRNA at 4 hpi with the wild-type (WT) and R2 mutant (R2). Where indicated, 9 μM of nocodazole was added 1 hr prior to infection and maintained throughout the infection. All values were plotted relative to levels observed during the untreated wild-type (WT) infection. Three independent experiments were performed with each experimental replicate performed in triplicate. Values are expressed as mean ± s.e.m. (****, p < 0.0001; ns, not significant based on two-tailed unpaired <i>t</i> test).</p> herpes simplex virus type;axonal transport;HSV;ganglia;axonal transport mechanism;varicella zoster virus;axonal transport deficit;pUL 37 tegument protein;time-lapse fluorescence microscopy;PRV;epithelial cell lines;intracellular capsid motion;herpes simplex virus type 1;pUL 37 tegument protein guides alpha-herpesvirus;trans-synaptic mapping studies 2017-12-07
    https://plos.figshare.com/articles/figure/Mutation_of_PRV_R2_results_in_a_post-entry_microtubule-dependent_delay_in_gene_expression_/5682427
10.1371/journal.ppat.1006741.g002