The GXXXG motif is dispensable for viral spread in tetherin-positive cells.
Constantin Brinkmann
Markus Hoffmann
Anastasia Lübke
Inga Nehlmeier
Annika Krämer-Kühl
Michael Winkler
Stefan Pöhlmann
10.1371/journal.pone.0189073.g006
https://plos.figshare.com/articles/figure/The_GXXXG_motif_is_dispensable_for_viral_spread_in_tetherin-positive_cells_/5682355
<p>(A) HeLa cells were transfected with the indicated siRNAs and subsequently infected with VSV wt or LXXXL mutant at an MOI of 0.005 for 1 h. Virus titers in culture supernatants were determined at the indicated time points post infection. The results of a single representative experiment carried out with triplicate samples are shown and were confirmed in two separate experiments. (B) The experiment was carried out as described for panel (A) but relative titers measured at 12 h post infection are shown. The results represent the average of three independent experiments performed with triplicate samples. Titers measured for untransfected control cells were set to 1. Error bars indicate standard error of the mean (SEM). (C) Vero cells stably expressing human tetherin (Vero-Tetherin) or stably containing empty vector (Vero) were infected with VSV wt or mutant LXXXL. Viral titers in culture supernatants were determined at the indicated time points post infection. The results of a single representative experiment carried out with triplicate samples are shown and were confirmed in two separate experiments. (D) The experiment was carried out as described for panel (C) but relative titers measured at 12 h post infection are shown. The results represent the average of three independent experiments performed with triplicate samples. Titers obtained from the respective control Vero cells were set to 1. Error bars indicate SEM. One-way ANOVA with Bonferroni post-test analyses were performed (B and D) to test statistical significance between selected groups (***, p ≤ 0.001).</p>
2017-12-07 18:57:58
transfected cells
tetherin-positive cells Vesicular stomatitis virus
VSV-G
IFN
host cell factor tetherin
HIV
BST
GXXXG motif
virus-encoded tetherin antagonist
VSV
viruses encode tetherin antagonists