ACT induces astrocyte damage.
Jonghyuk Park
Takahiro Masaki
Yoshihiro Mezaki
Hiroshi Yokoyama
Mariko Nakamura
Haruka Maehashi
Takahiko J. Fujimi
Sabine S. Gouraud
Keisuke Nagatsuma
Madoka Nakagomi
Naofumi Kimura
Tomokazu Matsuura
10.1371/journal.pone.0189346.g002
https://plos.figshare.com/articles/figure/ACT_induces_astrocyte_damage_/5682343
<p>(A) Astrocyte cells were co-stained with Hoechst 33342 and propidium iodide (PI). Cells were treated with NH<sub>4</sub>Cl (5 mM) and ACT (0.5 mg/ml) for 48 h. Final observation was conducted by fluorescence microscopy. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst-stained cells. Cells stained by PI represent dead cells, whereas Hoechst 33342 staining reveals all nuclei. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p>
2017-12-07 18:58:10
LPS
AVP receptor antagonist
EC
proteome analysis
Methods Mini-pig plasma samples
novel HE-inducible factors
ALF
astrocyte injury
BAL
hepatic encephalopathy Background