ACT induces astrocyte damage. Jonghyuk Park Takahiro Masaki Yoshihiro Mezaki Hiroshi Yokoyama Mariko Nakamura Haruka Maehashi Takahiko J. Fujimi Sabine S. Gouraud Keisuke Nagatsuma Madoka Nakagomi Naofumi Kimura Tomokazu Matsuura 10.1371/journal.pone.0189346.g002 https://plos.figshare.com/articles/figure/ACT_induces_astrocyte_damage_/5682343 <p>(A) Astrocyte cells were co-stained with Hoechst 33342 and propidium iodide (PI). Cells were treated with NH<sub>4</sub>Cl (5 mM) and ACT (0.5 mg/ml) for 48 h. Final observation was conducted by fluorescence microscopy. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst-stained cells. Cells stained by PI represent dead cells, whereas Hoechst 33342 staining reveals all nuclei. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p> 2017-12-07 18:58:10 LPS AVP receptor antagonist EC proteome analysis Methods Mini-pig plasma samples novel HE-inducible factors ALF astrocyte injury BAL hepatic encephalopathy Background