TY - DATA T1 - Alpha-1 antichymotrypsin is involved in astrocyte injury in concert with arginine-vasopressin during the development of acute hepatic encephalopathy - Fig 1 PY - 2017/12/07 AU - Jonghyuk Park AU - Takahiro Masaki AU - Yoshihiro Mezaki AU - Hiroshi Yokoyama AU - Mariko Nakamura AU - Haruka Maehashi AU - Takahiko J. Fujimi AU - Sabine S. Gouraud AU - Keisuke Nagatsuma AU - Madoka Nakagomi AU - Naofumi Kimura AU - Tomokazu Matsuura UR - https://plos.figshare.com/articles/figure/Alpha-1_antichymotrypsin_is_involved_in_astrocyte_injury_in_concert_with_arginine-vasopressin_during_the_development_of_acute_hepatic_encephalopathy_-_Fig_1/5682328 DO - 10.1371/journal.pone.0189346.g001 L4 - https://ndownloader.figshare.com/files/9940990 KW - LPS KW - AVP receptor antagonist KW - EC KW - proteome analysis KW - Methods Mini-pig plasma samples KW - novel HE-inducible factors KW - ALF KW - astrocyte injury KW - BAL KW - hepatic encephalopathy Background N2 - The growth and viability of human astrocytes cultured with plasma from mini-pigs with HE and the identification of candidate causative factors for HE by a 2-DE-based proteomic analysis (A) Experimental design: Mini-pig plasma was collected before the administration of α-amanitin and LPS, when encephalopathy occurred after the addition of toxins, and after treatment with extracorporeal circulation. (B) Astrocytes were cultured in medium with the collected plasma for 24 h. (C) Representative 2-DE gel images of silver-stained plasma proteins. These were based on LC-MS/MS data obtained from the NCBInr database using the MASCOT searching program. The labels 1 to 7 indicate the seven proteins that were upregulated before BAL treatment in comparison to normal plasma and which were downregulated after BAL treatment in comparison to before BAL treatment. (D) Astrocytes were incubated with various concentrations of ACT (0.01–0.5 mg/ml) for 24 h. Cell nuclei were counterstained with Hoechst 33342 with wavelengths of 350 nm excitation and 460 nm emission. Bars indicate the mean ± standard error of the mean (n = 5). *p < 0.05, in comparison to control (normal plasma). ER -