TY - DATA T1 - Cut-off value for absolute lymphocytes as an alternative for the immunophenotypic analysis of CD3+ T cells in the monitoring of immunosuppressive therapy with thymoglobulin PY - 2017/12/05 AU - Gislaine Furlanetto AU - Ana Paula Alegretti AU - Mariela Granero Farias AU - Priscila Aparecida Corrêa Freitas AU - Gustavo Muller Lara AU - Fabiane Spagnol Pedrazzani UR - https://scielo.figshare.com/articles/dataset/Cut-off_value_for_absolute_lymphocytes_as_an_alternative_for_the_immunophenotypic_analysis_of_CD3_T_cells_in_the_monitoring_of_immunosuppressive_therapy_with_thymoglobulin/5670547 DO - 10.6084/m9.figshare.5670547.v1 L4 - https://ndownloader.figshare.com/files/35534307 L4 - https://ndownloader.figshare.com/files/35534310 L4 - https://ndownloader.figshare.com/files/35534313 L4 - https://ndownloader.figshare.com/files/35534316 L4 - https://ndownloader.figshare.com/files/35534319 L4 - https://ndownloader.figshare.com/files/35534322 L4 - https://ndownloader.figshare.com/files/35534325 L4 - https://ndownloader.figshare.com/files/35534328 KW - flow cytometry KW - kidney transplantation KW - immunophenotyping KW - immunosuppressive agents N2 - Abstract Introduction: Immunosuppression of T lymphocytes is required for preventing acute rejection after transplantation and for the treatment of chronic autoimmune and inflammatory diseases. The laboratory monitoring for this therapy is the measurement of T cells by immunophenotyping, aiming the target value of less than 20 cells per µL. Objective: To establish a cut-off point for the total number of lymphocytes in the automated blood cell count that reflects less than twenty T cells µL by immunophenotyping. Methods: We studied and evaluated 242 kidney transplant patients that had results of automated blood cell count and quantification of T cells by immunophenotyping technique. The patients were divided into two groups, depending on the T lymphocyte immunophenotyping rates established by lower and higher than 20 cells per µL. After, we evaluated the cut-off point for lymphocytes in the blood cell count with a specificity of 100% to exclude patients with high levels of T lymphocytes. Results: We found that the cut-off point of 70 lymphocytes per µL obtained by automated blood cell count showed 100% of specificity to exclude patients with T-cell counts higher than 20 cells per µL by immunophenotyping. Conclusion: The results found in this study may be helpful to monitor the immunosuppressive therapy in kidney transplant patients in places where a flow cytometer is not available, or when this equipment is not present in the full routine. ER -