10.6084/m9.figshare.5669728.v1
Gabriela Fredo
Gabriela
Fredo
Ronaldo Viana Leite-Filho
Ronaldo Viana
Leite-Filho
Camila De Ávila Pietzsch
Camila De Ávila
Pietzsch
Caroline Pinto de Andrade
Caroline Pinto de
Andrade
Naila Cristina Blatt Duda
Naila Cristina Blatt
Duda
Lismara Castro do Nascimento
Lismara Castro do
Nascimento
Stella de Faria Valle
Stella de Faria
Valle
João Fábio Soares
João
Fábio Soares
Luciana Sonne
Luciana
Sonne
Rangeliosis: histopathological analysis, hematology and molecular detection of canine Rangelia vitalii in Rio Grande do Sul, Brazil
SciELO journals
2017
canids
hemoparasite
nambyuvú
Rangelia vitalii
rangeliosis
2017-12-05 12:55:49
Figure
https://scielo.figshare.com/articles/figure/Rangeliosis_histopathological_analysis_hematology_and_molecular_detection_of_canine_Rangelia_vitalii_in_Rio_Grande_do_Sul_Brazil/5669728
<div><p>ABSTRACT: In the period from January 2004 to December 2015, 56 dogs were diagnosed with rangeliosis in the Setor de Patologia Veterinária at Universidade Federal do Rio Grande do Sul (SPV-UFRGS). The main hematological abnormalities were thrombocytopenia and anemia. The affected dogs showed signs of apathy, anorexia, fetid and bloody diarrhea, vomiting, and dehydration. At necropsy, the main changes were jaundice, splenomegaly, hepatomegaly, and lymphadenomegaly. Histological analyses revealed parasitophorous vacuoles of Rangelia vitalii in cytoplasmic endothelial cells, mainly in the heart, kidneys, lymph nodes, intestines, and pancreas. Inflammation characterized by mononuclear cells was predominant in the analysis, and most was due to the presence of plasma cells. Other lesion types observed were lymphoid hyperplasia, extramedullary hematopoiesis, erythrophagocytosis, and erythroid lineage hyperplasia in bone marrow. Of the total number of animals, 49 were diagnosed using necropsy and histological analysis, and seven were diagnosed using a molecular analysis (i.e., PCR and genetic sequencing of blood samples). This paper presented a different method of diagnosing rangeliosis in canines. This approach involved histological methods including the quantification and determination of the intensity and distribution of the infectious agent in different organs.</p></div>