10.1371/journal.pone.0005845.g005
Elke Bayha
Elke
Bayha
Mette C. Jørgensen
Mette
C. Jørgensen
Palle Serup
Palle
Serup
Anne Grapin-Botton
Anne
Grapin-Botton
Electroporation of dominant negative RARs abolishes <i>CdxA</i> expression and pancreas formation.
Public Library of Science
2009
rars
abolishes
pancreas
2009-06-10 01:54:19
Figure
https://plos.figshare.com/articles/figure/_Electroporation_of_dominant_negative_RARs_abolishes_CdxA_expression_and_pancreas_formation_/566859
<p>Electroporation of pCIG (A,B,G,I,K) or pCIG-DNRAR (C–F,H,J,L). Whole mount <i>in situ</i> hybridization on stage HH 19 embryos shows <i>CdxA</i> expression (blue) in the closed duodenum and the open midgut. <i>CdxA</i> expression is repressed either partially (n = 7/11, C) or completely (n = 3/11, F) by DN-RAR. Cells expressing the expression construct are labeled by subsequent immunocytochemistry for GFP expressed from the bicistronic construct (Green in D, F and H, masked by blue <i>CdxA</i> staining in B, DAB-brown in G), demonstrating that repression extends to the neighbors of targeted cells. Whole mount immunocytochemistry on stage HH 20 embryos shows that dominant negative RAR (traced with GFP, green) represses pancreas progenitor emergence (traced with Nkx6.1, red) in a non-cell autonomous manner (J) as compared to control embryos electroporated with empty vector (I). Glucagon+ cells could still differentiate (blue). (K and L) Selected optical sections of embryos displayed in (I) and (J), respectively. Scale bar 200 µm.</p>