TY - DATA T1 - Plasminogen activator inhibitor 1 and Antipain preserve acrosome integrity of bovine spermatozoa during cryopreservation PY - 2017/12/05 AU - M.A. Castelo Branco AU - Y.N.T.C. Castelo Branco AU - F.J. Moraes Junior AU - F.N. Barros AU - F.P.S. Barçante AU - G.M.C. Carvalho AU - L.S. Melo Evangelista AU - A.L. Abreu-Silva AU - M.A. Sousa Filho AU - J.A.T. Souza UR - https://scielo.figshare.com/articles/dataset/Plasminogen_activator_inhibitor_1_and_Antipain_preserve_acrosome_integrity_of_bovine_spermatozoa_during_cryopreservation/5667859 DO - 10.6084/m9.figshare.5667859.v1 L4 - https://ndownloader.figshare.com/files/9898912 L4 - https://ndownloader.figshare.com/files/9898918 L4 - https://ndownloader.figshare.com/files/9898921 L4 - https://ndownloader.figshare.com/files/9898924 L4 - https://ndownloader.figshare.com/files/9898927 L4 - https://ndownloader.figshare.com/files/9898930 KW - Curraleiro Pé-Duro KW - fertility KW - seminal evaluation KW - serine protease inhibitors KW - sperm cryopreservation N2 - ABSTRACT Seminal plasma contains serine proteases and serine protease inhibitor, which are involved in mammalian fertilization, and the inhibitors can be applied to prevent cold-induced sperm capacitation. The effects of different concentrations of two serine protease inhibitors were analyzed, Plasminogen activator inhibitor 1 - PAI-1 (70ƞg, 140ƞg and 210 ƞg) and Antipain (10µg, 50µg and 100µg) as supplementation to bovine semen cryopreservation extender. The effects of the inhibitors on the sperm parameters (sperm kinetics - CASA, acrosome integrity, plasma membrane integrity, mitochondrial membrane potential, sperm defects and acrosome reaction rate) were evaluated in the post-thaw semen. Cryopreservation of sperm with Antipain decreased post-thaw kinetic parameters of MP, VSL, LIN, SRT and the percentage of hyper-activated sperm while PAI-1 (210 ƞg) decreased VSL and LIN. Antipain and PAI-1 had no effect on the integrity parameters of the plasma membrane, mitochondrial membrane potential and sperm defects. Sperm cryopreserved in the presence of Antipain and PAI-1 (70 and 140 ƞg) preserved acrosome integrity, as they were able to complete the in vitro acrosome reaction. In conclusion, the serine protease inhibitors, Antipain and PAI-1 (70 and 140ƞg) are able to preserve the acrosome integrity of cryopreserved bovine sperm. ER -