Vieira, Ana Elisa de Mello Danelon, Marcelle Camara, Danielle Mendes da Rosselli, Eliana Rodrigues Stock, Stuart R Cannon, Mark L Xiao, Xianghui De Carlo, Francesco Delbem, Alberto Carlos Botazzo In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization <div><p>Abstract Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.</p></div> X-ray microtomography;Tooth remineralization;Calcium phosphates;Fluoride;Dental enamel 2017-12-05
    https://scielo.figshare.com/articles/dataset/In_vitro_effect_of_amorphous_calcium_phosphate_paste_applied_for_extended_periods_of_time_on_enamel_remineralization/5667832
10.6084/m9.figshare.5667832.v1