10.6084/m9.figshare.5667469.v1 Vinicius Marques Antunes Ribeiro Vinicius Marques Antunes Ribeiro Aytube Lucas Coaglio Aytube Lucas Coaglio Fernando Luiz Pereira Oliveira Fernando Luiz Pereira Oliveira Cíntia Aparecida de Jesus Pereira Cíntia Aparecida de Jesus Pereira Walter dos Santos Lima Walter dos Santos Lima Dynamics of haemocytes from Pseudosuccinea columella circulating infected by Fasciola hepatica SciELO journals 2017 Fasciola hepatica haemocytes Pseudosuccinea columella 2017-12-05 09:12:43 Dataset https://scielo.figshare.com/articles/dataset/Dynamics_of_haemocytes_from_Pseudosuccinea_columella_circulating_infected_by_Fasciola_hepatica/5667469 <div><p>Abstract The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been observed at 30 minutes post-infection and 1, 7, 10, 14, 21, 28 and 50 days post-infection (dpi). Miracidia were observed head-foot and mantle at 30 minutes post-infection. Miracidia/Sporocysts in the mantle skirt 1 dpi, and fully formed sporocysts were observed in the head-foot at 7 dpi. Rediae became evident at 10 dpi and were located between the haemocoel and the muscles from 14 dpi; 50 dpi, the rediae in the digestive gland contained cercariae. The statistical analysis of the total haemocytes of P. columella infected by F. hepatica showed significant differences on the 30 minutes post-infection and 1, 14, 21, and 28 dpi in comparison to uninfected molluscs (0 dpi). Therefore, the interference observed on the internal defence system of P. columella may have direct association with the development of F. hepatica.</p></div>