TY - DATA T1 - Study of Microbial Interaction Formed by "Candida krusei" and "Candida glabrata": "In Vitro" and "In Vivo" Studies PY - 2017/12/05 AU - Rodnei Dennis Rossoni AU - Patrícia Pimentel de Barros AU - Fernanda Freire AU - Jéssica Diane dos Santos AU - Antonio Olavo Cardoso Jorge AU - Juliana Campos Junqueira UR - https://scielo.figshare.com/articles/dataset/Study_of_Microbial_Interaction_Formed_by_Candida_krusei_and_Candida_glabrata_In_Vitro_and_In_Vivo_Studies/5667325 DO - 10.6084/m9.figshare.5667325.v1 L4 - https://ndownloader.figshare.com/files/9895891 L4 - https://ndownloader.figshare.com/files/9895894 L4 - https://ndownloader.figshare.com/files/9895897 L4 - https://ndownloader.figshare.com/files/9895900 KW - Candida krusei KW - Candida glabrata KW - biofilms KW - experimental candidiasis KW - murine model N2 - Abstract Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay. For the in vivo study, twenty-four mice received topical applications of monospecie or mixed suspensions of each strain. After 48 h, yeasts were recovered from the mice and quantified by CFU/mL count. In the biofilm assays, the results for the CFU/mL count and the XTT assay showed that the two species studied were capable of forming high levels of in vitro monospecie biofilm. In mixed biofilm, the CFU of C. krusei increased (p=0.0001) and C. glabrata decreased (p=0.0001). The metabolic activity observed in XTT assay of mixed biofilm was significantly reduced compared with a single C. glabrata biofilm (p=0.0001). Agreeing with CFU in vitro count, C. glabrata CFU/mL values recovered from oral cavity of mice were statistically higher in the group with single infection (p=0.0001) than the group with mixed infection. We concluded that C. krusei inhibits C. glabrata and takes advantage to colonize the oral cavity and to form biofilms. ER -