10.6084/m9.figshare.5666935.v1
LUZIA YURIKO MIYATA
LUZIA YURIKO
MIYATA
RICARDO HARAKAVA
RICARDO
HARAKAVA
LÍSIA BORGES ATTÍLIO
LÍSIA BORGES
ATTÍLIO
BEATRIZ MADALENA JANUZZI MENDES
BEATRIZ MADALENA JANUZZI
MENDES
JOÃO ROBERTO SPOTTI LOPES
JOÃO ROBERTO SPOTTI
LOPES
HELVÉCIO DELLA COLETTA-FILHO
HELVÉCIO DELLA
COLETTA-FILHO
ALESSANDRA ALVES DE SOUZA
ALESSANDRA ALVES DE
SOUZA
FRANCISCO DE ASSIS ALVES MOURÃO FILHO
FRANCISCO DE ASSIS ALVES
MOURÃO FILHO
PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus
SciELO journals
2017
Citrus sinensis
Diaphorina citri
genetic transformation
GUS
huanglongbing
quantitative real-time PCR
2017-12-05 08:55:43
Dataset
https://scielo.figshare.com/articles/dataset/PHLOEM_PROMOTERS_IN_TRANSGENIC_SWEET_ORANGE_ARE_DIFFERENTIALLY_TRIGGERED_BY_Candidatus_Liberibacter_asiaticus/5666935
<div><p>ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las.</p></div>