TY - DATA T1 - The effects of local ischemic preconditioning and topical hypothermia in renal ischemia/reperfusion injury in rats PY - 2017/12/05 AU - Guilherme Behrend Ribeiro AU - Emanuel Burck dos Santos AU - Silvia Regina Bona AU - Pedro Guilherme Schaefer AU - Tuane Alves Garcez AU - Eduardo Brasil Rabolini AU - Guilherme Pereira Smaniotto AU - Norma Possa Marroni AU - Carlos Otávio Corso UR - https://scielo.figshare.com/articles/dataset/The_effects_of_local_ischemic_preconditioning_and_topical_hypothermia_in_renal_ischemia_reperfusion_injury_in_rats/5666572 DO - 10.6084/m9.figshare.5666572.v1 L4 - https://ndownloader.figshare.com/files/9890893 L4 - https://ndownloader.figshare.com/files/9890899 L4 - https://ndownloader.figshare.com/files/9890902 L4 - https://ndownloader.figshare.com/files/9890911 L4 - https://ndownloader.figshare.com/files/9890920 L4 - https://ndownloader.figshare.com/files/9890938 L4 - https://ndownloader.figshare.com/files/9890944 L4 - https://ndownloader.figshare.com/files/9890947 L4 - https://ndownloader.figshare.com/files/9890950 L4 - https://ndownloader.figshare.com/files/9890953 KW - Ischemic Preconditioning KW - Hypothermia KW - Reperfusion Injury KW - Oxidative Stress KW - Kidney KW - Rats N2 - Abstract Purpose: Topical hypothermia and local ischemic preconditioning have been shown to reduce renal ischemia-reperfusion (I/R) injury individually. We examined whether combination of both strategies lessens renal I/R injury. Methods: Post right nephrectomy, 40 male Wistar rats were randomly assigned to five experimental protocols performed in the left kidney: topical hypothermia without ischemia (TH), warm ischemia (IR), ischemic preconditioning followed by warm ischemia (IPC+IR), cold ischemia (TH+IR), and ischemic preconditioning followed by cold ischemia (IPC+TH+IR). Eight randomly assigned right kidneys constituted the control group. After 240 min of reperfusion, the left kidney was retrieved to evaluate histological changes, lipid peroxidation and antioxidant enzymes activity. Serum was collected to evaluate urea and creatinine. Results: IPC+TH+IR group revealed no difference to any other group subjected to ischemia in relation to histological changes, lipid peroxidation and antioxidant enzymes activity. Creatinine was lower in IPC+TH+IR group compared with IPC+IR, but showed no difference compared to TH+IR group. Conclusions: Combination of local ischemic preconditioning (IPC) and topical hypothermia conferred no protection in renal I/R injury. Moreover, local IPC solely followed by warm ischemia impaired renal function more than warm ischemia alone. ER -