TY - DATA T1 - Interferon-inducible Mx1 protein is highly expressed in renal tissues from treatment-naïve lupus nephritis, but not in those under immunosuppressive treatment PY - 2017/11/30 AU - Yuka Shimizu AU - Shinsuke Yasuda AU - Taichi Kimura AU - Saori Nishio AU - Michihiro Kono AU - Kazumasa Ohmura AU - Sanae Shimamura AU - Michihito Kono AU - Yuichiro Fujieda AU - Masaru Kato AU - Kenji Oku AU - Toshiyuki Bohgaki AU - Yuichiro Fukasawa AU - Shinya Tanaka AU - Tatsuya Atsumi UR - https://tandf.figshare.com/articles/figure/Interferon-inducible_Mx1_protein_is_highly_expressed_in_renal_tissues_from_treatment-na_ve_lupus_nephritis_but_not_in_those_under_immunosuppressive_treatment/5649565 DO - 10.6084/m9.figshare.5649565.v1 L4 - https://ndownloader.figshare.com/files/9851986 KW - Systemic lupus erythematosus KW - lupus nephritis KW - type I interferon KW - Mx1 N2 - Objectives: The aim of this study was to clarify the consequences of Mx1, one of the IFN-inducible proteins, in the peripheral blood as well as in renal tissues in patients with systemic lupus erythematosus (SLE). Patients and methods: Mx1 protein concentrations in (PBMCs) from 18 SLE patients mostly in their stable disease status, 11 IgA nephropathy (IgAN) patients, 5 ANCA-associated vasculitis (AAV) patients and 16 healthy controls were measured using enzyme-linked immunosorbent assay (ELISA). Mx1 expression in renal specimens from 18 patients with lupus nephritis (LN), 18 with IgAN and 10 with AAV were evaluated using immunohistochemistry. Results: Mx1 protein concentrations in lysates of PBMCs were significantly higher in SLE patients compared with those in other three groups. Mx1-positive area in renal tissues was significantly dominant in both glomeruli and renal tubules of LN compared with other renal diseases. Renal Mx1 protein levels were lower in LN after immunosuppressive treatment, compared with those from immunosuppressant-naïve patients. Conclusion: Mx1 levels were upregulated in lupus peripheral blood even when their disease activities were stable. On the other hand, Mx1 was highly expressed in kidneys from patients with LN before treatment, which was decreased after immunosuppressive treatment. These results suggest that Mx1 is a potential marker for the diagnosis of SLE in the peripheral blood and also for the activity of lupus nephritis in the kidney. ER -