10.6084/m9.figshare.5635000 Ricardo P. Santos Ricardo P. Santos Luana M. Souza Luana M. Souza Acenini L. Balieiro Acenini L. Balieiro Cleide M. F. Soares Cleide M. F. Soares Álvaro S. Lima Álvaro S. Lima Ranyere L. Souza Ranyere L. Souza Integrated process of extraction and purification of betanin from <i>Opuntia ficus-indica</i> using aqueous two-phase systems based on THF and sodium salts Taylor & Francis Group 2017 Opuntia ficus-indica betanin extraction purification aqueous two-phase systems 2017-11-27 15:11:24 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Integrated_process_of_extraction_and_purification_of_betanin_from_i_Opuntia_ficus-indica_i_using_aqueous_two-phase_systems_based_on_THF_and_sodium_salts/5635000 <p>This research present an integrated process for the extraction and purification of betanin from fruit of <i>Opuntia ficus-indica</i>. The extraction process was evaluated using different solvents (water, acetonitrile, ethanol and tetrahydrofuran), and then integrated into the purification process using aqueous two-phase systems (ATPS). The optimum conditions for extraction was with THF and the purification conditions determined to be 43 wt% of THF and 6 wt% of sodium citrate buffer (pH 5.5) at 25°C. A purification factor of 13.7 ± 0.5 fold, with a partition coefficient of betanin for salt-rich phase and the antioxidant capacity was increased ≈ 65%.</p>