10.6084/m9.figshare.5635000
Ricardo P. Santos
Ricardo P.
Santos
Luana M. Souza
Luana M.
Souza
Acenini L. Balieiro
Acenini L.
Balieiro
Cleide M. F. Soares
Cleide M. F.
Soares
Álvaro S. Lima
Álvaro S.
Lima
Ranyere L. Souza
Ranyere L.
Souza
Integrated process of extraction and purification of betanin from <i>Opuntia ficus-indica</i> using aqueous two-phase systems based on THF and sodium salts
Taylor & Francis Group
2017
Opuntia ficus-indica
betanin
extraction
purification
aqueous two-phase systems
2017-11-27 15:11:24
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Integrated_process_of_extraction_and_purification_of_betanin_from_i_Opuntia_ficus-indica_i_using_aqueous_two-phase_systems_based_on_THF_and_sodium_salts/5635000
<p>This research present an integrated process for the extraction and purification of betanin from fruit of <i>Opuntia ficus-indica</i>. The extraction process was evaluated using different solvents (water, acetonitrile, ethanol and tetrahydrofuran), and then integrated into the purification process using aqueous two-phase systems (ATPS). The optimum conditions for extraction was with THF and the purification conditions determined to be 43 wt% of THF and 6 wt% of sodium citrate buffer (pH 5.5) at 25°C. A purification factor of 13.7 ± 0.5 fold, with a partition coefficient of betanin for salt-rich phase and the antioxidant capacity was increased ≈ 65%.</p>