10.6084/m9.figshare.5634886 Letícia Pereira Pimenta Letícia Pereira Pimenta Luís Claúdio Kellner Filho Luís Claúdio Kellner Filho Bruna Arruda Soares Parpinelli Bruna Arruda Soares Parpinelli Katia Aparecida de Siqueira Katia Aparecida de Siqueira Marcos Antônio Soares Marcos Antônio Soares Márcio Luís Andrade e Silva Márcio Luís Andrade e Silva Wilson Roberto Cunha Wilson Roberto Cunha Patrícia Mendonça Pauletti Patrícia Mendonça Pauletti Ana Helena Januário Ana Helena Januário DEVELOPMENT AND VALIDATION OF AN HPLC-DAD ANALYTICAL METHOD TO QUANTIFY 5-METHOXYFLAVONES IN METHANOLIC EXTRACTS OF Vochysia divergens POHL CULTURED UNDER STRESS CONDITIONS SciELO journals 2017 Vochysia divergens 5-methoxyflavones HPLC-DAD quantification water stress endophytic fungi 2017-11-29 11:27:22 Dataset https://scielo.figshare.com/articles/dataset/DEVELOPMENT_AND_VALIDATION_OF_AN_HPLC-DAD_ANALYTICAL_METHOD_TO_QUANTIFY_5-METHOXYFLAVONES_IN_METHANOLIC_EXTRACTS_OF_Vochysia_divergens_POHL_CULTURED_UNDER_STRESS_CONDITIONS/5634886 <div><p>Vochysia divergens Pohl, known as "Cambara" in Brazil, is an invasive species that is expanding throughout Pantanal in Brazil, to form mono-dominant communities. This expansion is affecting the agricultural areas that support the typical seasonal flood and drought conditions of this biome. This article describes the development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of greenhouse-grown V. divergens associated with one of two endophytic fungal species Zopfiella tetraspora (Zt) or Melanconiella elegans (Me) and later subjected to water stress. The developed method gave good validation parameters and was successfully applied to quantify the flavones 3',5-dimethoxy luteolin-7-O-β-glucopyranoside (1), 5-methoxy luteolin (2), and 3',5-dimethoxy luteolin (3) in the target extracts. Inoculation of the plant with Zt decreased the concentration of flavone 1 in the extract by 2.69-fold as compared to the control. Inoculation of the plant with Zt or Me did not significantly alter the contents of flavones 2 and 3 in the extracts as compared to the control. Therefore, the aerial parts of germinated V. divergens plants inoculated with either Zt or Me responded differently in terms of the production of flavones. These results can cast light on the symbiosis between fungal microorganisms and V. divergens, which most likely influences the response of V. divergens to changes in the availability of water in Pantanal.</p></div>