10.6084/m9.figshare.5634886
Letícia Pereira Pimenta
Letícia Pereira
Pimenta
Luís Claúdio Kellner Filho
Luís Claúdio
Kellner Filho
Bruna Arruda Soares Parpinelli
Bruna Arruda Soares
Parpinelli
Katia Aparecida de Siqueira
Katia Aparecida de
Siqueira
Marcos Antônio Soares
Marcos Antônio
Soares
Márcio Luís Andrade e Silva
Márcio Luís Andrade e
Silva
Wilson Roberto Cunha
Wilson Roberto
Cunha
Patrícia Mendonça Pauletti
Patrícia Mendonça
Pauletti
Ana Helena Januário
Ana Helena
Januário
DEVELOPMENT AND VALIDATION OF AN HPLC-DAD ANALYTICAL METHOD TO QUANTIFY 5-METHOXYFLAVONES IN METHANOLIC EXTRACTS OF Vochysia divergens POHL CULTURED UNDER STRESS CONDITIONS
SciELO journals
2017
Vochysia divergens
5-methoxyflavones
HPLC-DAD quantification
water stress
endophytic fungi
2017-11-29 11:27:22
Dataset
https://scielo.figshare.com/articles/dataset/DEVELOPMENT_AND_VALIDATION_OF_AN_HPLC-DAD_ANALYTICAL_METHOD_TO_QUANTIFY_5-METHOXYFLAVONES_IN_METHANOLIC_EXTRACTS_OF_Vochysia_divergens_POHL_CULTURED_UNDER_STRESS_CONDITIONS/5634886
<div><p>Vochysia divergens Pohl, known as "Cambara" in Brazil, is an invasive species that is expanding throughout Pantanal in Brazil, to form mono-dominant communities. This expansion is affecting the agricultural areas that support the typical seasonal flood and drought conditions of this biome. This article describes the development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of greenhouse-grown V. divergens associated with one of two endophytic fungal species Zopfiella tetraspora (Zt) or Melanconiella elegans (Me) and later subjected to water stress. The developed method gave good validation parameters and was successfully applied to quantify the flavones 3',5-dimethoxy luteolin-7-O-β-glucopyranoside (1), 5-methoxy luteolin (2), and 3',5-dimethoxy luteolin (3) in the target extracts. Inoculation of the plant with Zt decreased the concentration of flavone 1 in the extract by 2.69-fold as compared to the control. Inoculation of the plant with Zt or Me did not significantly alter the contents of flavones 2 and 3 in the extracts as compared to the control. Therefore, the aerial parts of germinated V. divergens plants inoculated with either Zt or Me responded differently in terms of the production of flavones. These results can cast light on the symbiosis between fungal microorganisms and V. divergens, which most likely influences the response of V. divergens to changes in the availability of water in Pantanal.</p></div>