10.6084/m9.figshare.5634799 Tania MENEGOL Tania MENEGOL Andressa Bacalau DIPRAT Andressa Bacalau DIPRAT Eliseu RODRIGUES Eliseu RODRIGUES Rosane RECH Rosane RECH Effect of temperature and nitrogen concentration on biomass composition of Heterochlorella luteoviridis SciELO journals 2017 nitrogen temperature lipids omega-3 carotenoids microalga 2017-11-29 11:22:49 Dataset https://scielo.figshare.com/articles/dataset/Effect_of_temperature_and_nitrogen_concentration_on_biomass_composition_of_Heterochlorella_luteoviridis/5634799 <div><p>Abstract The interest in microalga as a food supplement has grown due their high contents of carotenoids, polyunsaturated fatty acids and proteins. This study evaluated the effect of different temperatures (22, 27 or 32 °C) and sodium nitrate concentrations (12, 24, 36, 48 or 60 mg L-1 of N-NO3) in culture medium on Heterochlorella luteoviridis biomass production and composition. The highest biomass concentration (3.35 g L-1) was observed at the highest N-NO3 concentration. The N-NO3 concentration positively affected protein, carbohydrate and carotenoids contents of biomass. On the other hand, cells cultured at the lowest N-NO3 concentration showed a slight increment in lipid content. The major carotenoid was lutein (30.7 ± 1.4% of total carotenoids), and the polyunsaturated fatty acids were 37 ± 2% of total fatty acids. Low temperature improved the biosynthesis of ω3 type fatty acids by lowering the ω6:ω3 ratio. Overall, our results indicate H. luteoviridis can yield high biomass concentration under autotrophic growth, resulting in a biomass rich in carotenoids, mainly lutein, and ω3 polyunsaturated fatty acids.</p></div>