10.1371/journal.pone.0006206.g007 Francesca Pistollato Francesca Pistollato Elena Rampazzo Elena Rampazzo Sara Abbadi Sara Abbadi Alessandro Della Puppa Alessandro Della Puppa Renato Scienza Renato Scienza Domenico D'Avella Domenico D'Avella Luca Denaro Luca Denaro Geertruy te Kronnie Geertruy te Kronnie David M. Panchision David M. Panchision Giuseppe Basso Giuseppe Basso Exogenous BMP2 promotes increase of PHD2 protein level even under hypoxia by dowregulating FKBP38. Public Library of Science 2009 bmp2 phd2 hypoxia dowregulating 2009-07-09 00:55:00 Figure https://plos.figshare.com/articles/figure/_Exogenous_BMP2_promotes_increase_of_PHD2_protein_level_even_under_hypoxia_by_dowregulating_FKBP38_/563300 <p>(A,B) Representative western blot analyses of PHD2 and FKBP38; GBM-derived cells, initially expanded in 2% oxygen were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006206#pone-0006206-g004" target="_blank">Fig. 4</a>. Also, some GBM-derived cells were treated with 10 ng/ml BMP2 for longer time (72 hrs) (B). (C,E) Bar graphs showing mean intensity of PHD2 and FKBP38 proteins normalized to control at 2% oxygen (corresponding to the 0 base line)±S.E.M. comparing 6 different tumors, n = 3 for each tumor. (D,F) QRT-PCR analyses of <i>PHD2</i> and <i>FKBP38</i>, normalized to <i>GUSB</i> and then calibrated to 2% oxygen control (ΔΔCt Method), mean±S.E.M. comparing 3 different GBM, n = 3 for each tumor.</p>