10.1371/journal.pone.0006206.g007
Francesca Pistollato
Francesca
Pistollato
Elena Rampazzo
Elena
Rampazzo
Sara Abbadi
Sara
Abbadi
Alessandro Della Puppa
Alessandro
Della Puppa
Renato Scienza
Renato
Scienza
Domenico D'Avella
Domenico
D'Avella
Luca Denaro
Luca
Denaro
Geertruy te Kronnie
Geertruy
te Kronnie
David M. Panchision
David
M. Panchision
Giuseppe Basso
Giuseppe
Basso
Exogenous BMP2 promotes increase of PHD2 protein level even under hypoxia by dowregulating FKBP38.
Public Library of Science
2009
bmp2
phd2
hypoxia
dowregulating
2009-07-09 00:55:00
Figure
https://plos.figshare.com/articles/figure/_Exogenous_BMP2_promotes_increase_of_PHD2_protein_level_even_under_hypoxia_by_dowregulating_FKBP38_/563300
<p>(A,B) Representative western blot analyses of PHD2 and FKBP38; GBM-derived cells, initially expanded in 2% oxygen were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006206#pone-0006206-g004" target="_blank">Fig. 4</a>. Also, some GBM-derived cells were treated with 10 ng/ml BMP2 for longer time (72 hrs) (B). (C,E) Bar graphs showing mean intensity of PHD2 and FKBP38 proteins normalized to control at 2% oxygen (corresponding to the 0 base line)±S.E.M. comparing 6 different tumors, n = 3 for each tumor. (D,F) QRT-PCR analyses of <i>PHD2</i> and <i>FKBP38</i>, normalized to <i>GUSB</i> and then calibrated to 2% oxygen control (ΔΔCt Method), mean±S.E.M. comparing 3 different GBM, n = 3 for each tumor.</p>