10.6084/m9.figshare.5631880
Samuel G. Rouleau
Samuel G.
Rouleau
Jean-Michel Garant
Jean-Michel
Garant
François Bolduc
François
Bolduc
Martin Bisaillon
Martin
Bisaillon
Jean-Pierre Perreault
Jean-Pierre
Perreault
G-Quadruplexes influence pri-microRNA processing
Taylor & Francis Group
2017
Antisense Oligonucleotide
biogenesis
mir200c
mir451a
mir497
microRNA
G-Quadruplex
miRNA
pri-miRNA processing
2017-11-24 11:17:23
Dataset
https://tandf.figshare.com/articles/dataset/G-Quadruplexes_influence_pri-microRNA_processing/5631880
<p>RNA G-Quadruplexes (G4) have been shown to possess many biological functions, including the regulation of microRNA (miRNA) biogenesis and function. However, their impact on pri-miRNA processing remains unknown. We identified G4 located near the Drosha cleavage site in three distinct pri-miRNAs: pri-mir200c, pri-mir451a, and pri-mir497. The folding of the potential G4 motifs was determined in solution. Subsequently, mutations disrupting G4 folding led to important changes in the mature miRNAs levels in cells. Moreover, using small antisense oligonucleotides binding to the pri-miRNA, it was possible to modulate, either positively or negatively, the mature miRNA levels. Together, these data demonstrate that G4 motifs could contribute to the regulation of pri-mRNA processing, a novel role for G4. Considering that bio-informatics screening indicates that between 9% and 50% of all pri-miRNAs contain a putative G4, these structures possess interesting potential as future therapeutic targets.</p>