10.6084/m9.figshare.5625028.v1
Pisa J.H.
Pisa
J.H.
Manfredi A.P.
Manfredi
A.P.
Perotti N.I.
Perotti
N.I.
Romero H.G.
Romero
H.G.
Breccia J.D.
Breccia
J.D.
Martínez M.A.
Martínez
M.A.
Supplementary Material for: Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
Karger Publishers
2017
Paenibacillaceae
Cohnella
Sugarcane bagasse
Hemicellulose degradation
2017-11-22 13:49:20
Journal contribution
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Agrowastes_as_Feedstock_for_the_Production_of_Endo-_-Xylanase_from_Cohnella_sp_Strain_AR92/5625028
<p>Members of <i>Cohnella </i>sp. isolated from a variety of
environments have been shown to be glycoside hydrolase producers.
Nevertheless, most evaluations of members of this genus are limited to
their taxonomic description. The strain AR92, previously identified as
belonging to the genus <i>Cohnella</i>, formed a well-supported cluster with <i>C. thailandensis</i> and <i>C. formosensis</i>
(>80% bootstrap confidence). Its growth and xylanase production were
approached by using a mineral-based medium containing alkali-pretreated
sugarcane bagasse as the main carbon source, which was assayed as a
convenient source to produce biocatalysts potentially fitting its
degradation. By means of a two-step statistical approach, the production
of endoxylanase was moderately improved (20%). However, a far more
significant improvement was observed (145%), by increasing the inoculum
size and lowering the fermentation temperature to 25°C, which is below
the optimal growth temperature of the strain AR92 (37°C). The
xylanolytic preparation produced by <i>Cohnella</i> sp. AR92 contained
mild temperature-active endoxylanase (identified as redundant GH10
family) for the main activity which resulted in xylobiose and
xylo-oligosaccharides as the main products from birchwood xylan.</p>