10.6084/m9.figshare.5624593
Kazutoshi Shindo
Kazutoshi
Shindo
Jun-ichiro Hattan
Jun-ichiro
Hattan
Mariko Kato
Mariko
Kato
Miho Sato
Miho
Sato
Tomoko Ito
Tomoko
Ito
Yurika Shibuya
Yurika
Shibuya
Arisa Watanabe
Arisa
Watanabe
Maki Sugiyama
Maki
Sugiyama
Yuri Nakamura
Yuri
Nakamura
Norihiko Misawa
Norihiko
Misawa
Purification and structural analysis of volatile sesquiterpenes produced by <i>Escherichia coli</i> carrying unidentified terpene synthase genes from edible plants of the family <i>Araliaceae</i>
Taylor & Francis Group
2017
Tps genes
Araliaceae
recombinant Escherichia coli
volatile sesquiterpene
purification and structural determination
2017-11-22 08:53:22
Dataset
https://tandf.figshare.com/articles/dataset/Purification_and_structural_analysis_of_volatile_sesquiterpenes_produced_by_i_Escherichia_coli_i_carrying_unidentified_terpene_synthase_genes_from_edible_plants_of_the_family_i_Araliaceae_i_/5624593
<p>A simple method to purify volatile sesquiterpenes from recombinant <i>Escherichia coli</i> was developed using the cells that carried known sesquiterpene synthase (<i>Tps</i>) genes <i>ZzZss</i>2 (<i>ZSS</i>2) and <i>ZoTps</i>1. This method was applied for the purification and structural analyses of volatile sesquiterpenes produced by <i>E. coli</i> cells that carried unidentified <i>Tps</i> genes, which were isolated from the <i>Aralia</i>-genus edible plants belonging to the family <i>Araliaceae</i>. Recombinant cells carrying each Tps gene were cultured in the two-layer medium (<i>n</i>-octane/TB medium), and volatile sesquiterpenes trapped in <i>n</i>-octane were purified through two-phase partition, silica gel column chromatography, and reversed-phase preparative high-performance liquid chromatography, if necessary. Further, their structures were confirmed by nuclear magnetic resonance, [α]<sub>D</sub>, and gas chromatography–mass spectrometry analyses. Herein, the products of <i>E. coli</i> cells that carried two <i>Tps</i> gene (named <i>AcTps</i>1 and <i>AcTps</i>2) in <i>Araria cordata</i> “Udo” and a <i>Tps</i> gene (named <i>AeTps</i>1) in <i>Aralia elata</i> “Taranoki” were studied resulting in identifying functionalities of these cryptic <i>Tps</i> genes.</p> <p>Purification and structural analysis of volatile sesquiterpenes produced by <i>Escherichia coli</i> carrying unidentified terpene synthase genes from edible plants of the family <i>Araliaceae</i>.</p>