10.6084/m9.figshare.5624593 Kazutoshi Shindo Kazutoshi Shindo Jun-ichiro Hattan Jun-ichiro Hattan Mariko Kato Mariko Kato Miho Sato Miho Sato Tomoko Ito Tomoko Ito Yurika Shibuya Yurika Shibuya Arisa Watanabe Arisa Watanabe Maki Sugiyama Maki Sugiyama Yuri Nakamura Yuri Nakamura Norihiko Misawa Norihiko Misawa Purification and structural analysis of volatile sesquiterpenes produced by <i>Escherichia coli</i> carrying unidentified terpene synthase genes from edible plants of the family <i>Araliaceae</i> Taylor & Francis Group 2017 Tps genes Araliaceae recombinant Escherichia coli volatile sesquiterpene purification and structural determination 2017-11-22 08:53:22 Dataset https://tandf.figshare.com/articles/dataset/Purification_and_structural_analysis_of_volatile_sesquiterpenes_produced_by_i_Escherichia_coli_i_carrying_unidentified_terpene_synthase_genes_from_edible_plants_of_the_family_i_Araliaceae_i_/5624593 <p>A simple method to purify volatile sesquiterpenes from recombinant <i>Escherichia coli</i> was developed using the cells that carried known sesquiterpene synthase (<i>Tps</i>) genes <i>ZzZss</i>2 (<i>ZSS</i>2) and <i>ZoTps</i>1. This method was applied for the purification and structural analyses of volatile sesquiterpenes produced by <i>E. coli</i> cells that carried unidentified <i>Tps</i> genes, which were isolated from the <i>Aralia</i>-genus edible plants belonging to the family <i>Araliaceae</i>. Recombinant cells carrying each Tps gene were cultured in the two-layer medium (<i>n</i>-octane/TB medium), and volatile sesquiterpenes trapped in <i>n</i>-octane were purified through two-phase partition, silica gel column chromatography, and reversed-phase preparative high-performance liquid chromatography, if necessary. Further, their structures were confirmed by nuclear magnetic resonance, [α]<sub>D</sub>, and gas chromatography–mass spectrometry analyses. Herein, the products of <i>E. coli</i> cells that carried two <i>Tps</i> gene (named <i>AcTps</i>1 and <i>AcTps</i>2) in <i>Araria cordata</i> “Udo” and a <i>Tps</i> gene (named <i>AeTps</i>1) in <i>Aralia elata</i> “Taranoki” were studied resulting in identifying functionalities of these cryptic <i>Tps</i> genes.</p> <p>Purification and structural analysis of volatile sesquiterpenes produced by <i>Escherichia coli</i> carrying unidentified terpene synthase genes from edible plants of the family <i>Araliaceae</i>.</p>