10.6084/m9.figshare.5615383.v1 Luca Campone Luca Campone Anna Lisa Piccinelli Anna Lisa Piccinelli Rita Celano Rita Celano Imma Pagano Imma Pagano Rosa Di Sanzo Rosa Di Sanzo Sonia Carabetta Sonia Carabetta Mariateresa Russo Mariateresa Russo Luca Rastrelli Luca Rastrelli Occurrence of aflatoxin M1 in milk samples from Italy analysed by online-SPE UHPLC-MS/MS Taylor & Francis Group 2017 Aflatoxin M1 mycotoxin analysis on-line SPE occurrence of aflatoxin M1 milk and milk samples 2017-11-18 07:57:50 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Occurrence_of_aflatoxin_M1_in_milk_samples_from_Italy_analysed_by_online-SPE_UHPLC-MS_MS/5615383 <p>The occurrence of aflatoxin M1 in 69 milk samples collected in a south region of Italy in 2016 was evaluated. The samples were analysed using an automated method based on online SPE coupled with UHPLC tandem mass spectrometry. After a salt induced liquid–liquid extraction with acetonitrile to remove protein from milk, the extract was diluted with water and analysed using an automated online SPE MS/MS method. Among the analysed samples no one had AFM1 higher than the legally allowable limits whereas 71.4% of the other analysed samples were above the LOD of the method. The highest contamination level of AFM1 was found in pasteurised milk (44.39 ng kg<sup>−1</sup>). The results show the worrying and widespread of AFM1 contamination, highlighting the necessity of monitoring studies in order to evaluate the reduction of the maximum legal limit.</p>