10.1371/journal.pone.0187901.g008 Hector Sanz Hector Sanz John J. Aponte John J. Aponte Jaroslaw Harezlak Jaroslaw Harezlak Yan Dong Yan Dong Aintzane Ayestaran Aintzane Ayestaran Augusto Nhabomba Augusto Nhabomba Maxmillian Mpina Maxmillian Mpina Obiang Régis Maurin Obiang Régis Maurin Núria Díez-Padrisa Núria Díez-Padrisa Ruth Aguilar Ruth Aguilar Gemma Moncunill Gemma Moncunill Agnandij Selidji Todagbe Agnandij Selidji Todagbe Claudia Daubenberger Claudia Daubenberger Carlota Dobaño Carlota Dobaño Clarissa Valim Clarissa Valim Standardized residuals as a function of the predicted base 10 logarithm median fluorescence intensity (MFI) for the four emulated datasets based on standard curves fitted after outliers were flagged and using the four different approaches to treat background noise. Public Library of Science 2017 quality control Suboptimal calibration fitness RTS method malaria vaccine multiplex bead-based immunoassays data analysis Multiplex bead-based immunoassays background noise multiplex bead-based immunoassay estimation function SC Phase 3 randomized sample quantification analyte concentration drLumi conduct quality control 2017-11-14 18:41:23 Figure https://plos.figshare.com/articles/figure/Standardized_residuals_as_a_function_of_the_predicted_base_10_logarithm_median_fluorescence_intensity_MFI_for_the_four_emulated_datasets_based_on_standard_curves_fitted_after_outliers_were_flagged_and_using_the_four_different_approaches_to_treat_backgroun/5600731 <p>Points outside of the range specified by the dashed lines are in red and labeled according to the well location in the 96-well plate. (A) Standard curve fitted using “Ignore” background noise method; (B) standard curve fitted including the background noise as an extra point when fitting the curve, using “Include” background method; (C) standard curve fitted subtracting the background noise using the “Subtract” method; (D) standard curve fitted constraining the lower asymptote to the background noise using the “Constraint” method.</p>