10.1371/journal.pone.0187901.g008
Hector Sanz
Hector
Sanz
John J. Aponte
John
J. Aponte
Jaroslaw Harezlak
Jaroslaw
Harezlak
Yan Dong
Yan
Dong
Aintzane Ayestaran
Aintzane
Ayestaran
Augusto Nhabomba
Augusto
Nhabomba
Maxmillian Mpina
Maxmillian
Mpina
Obiang Régis Maurin
Obiang Régis
Maurin
Núria Díez-Padrisa
Núria
Díez-Padrisa
Ruth Aguilar
Ruth
Aguilar
Gemma Moncunill
Gemma
Moncunill
Agnandij Selidji Todagbe
Agnandij Selidji
Todagbe
Claudia Daubenberger
Claudia
Daubenberger
Carlota Dobaño
Carlota
Dobaño
Clarissa Valim
Clarissa
Valim
Standardized residuals as a function of the predicted base 10 logarithm median fluorescence intensity (MFI) for the four emulated datasets based on standard curves fitted after outliers were flagged and using the four different approaches to treat background noise.
Public Library of Science
2017
quality control
Suboptimal calibration
fitness
RTS
method
malaria
vaccine
multiplex bead-based immunoassays data analysis Multiplex bead-based immunoassays
background noise
multiplex bead-based immunoassay
estimation
function
SC
Phase 3 randomized
sample
quantification
analyte
concentration
drLumi
conduct quality control
2017-11-14 18:41:23
Figure
https://plos.figshare.com/articles/figure/Standardized_residuals_as_a_function_of_the_predicted_base_10_logarithm_median_fluorescence_intensity_MFI_for_the_four_emulated_datasets_based_on_standard_curves_fitted_after_outliers_were_flagged_and_using_the_four_different_approaches_to_treat_backgroun/5600731
<p>Points outside of the range specified by the dashed lines are in red and labeled according to the well location in the 96-well plate. (A) Standard curve fitted using “Ignore” background noise method; (B) standard curve fitted including the background noise as an extra point when fitting the curve, using “Include” background method; (C) standard curve fitted subtracting the background noise using the “Subtract” method; (D) standard curve fitted constraining the lower asymptote to the background noise using the “Constraint” method.</p>