Cartoon showing the influence of the Subtract method into the standard samples. Hector Sanz John J. Aponte Jaroslaw Harezlak Yan Dong Aintzane Ayestaran Augusto Nhabomba Maxmillian Mpina Obiang Régis Maurin Núria Díez-Padrisa Ruth Aguilar Gemma Moncunill Agnandij Selidji Todagbe Claudia Daubenberger Carlota Dobaño Clarissa Valim 10.1371/journal.pone.0187901.g001 https://plos.figshare.com/articles/figure/Cartoon_showing_the_influence_of_the_Subtract_method_into_the_standard_samples_/5600683 <p>Background noise calculated as the geometric mean of the blank controls is shown as a horizontal dashed line. (A) Standard curve plotting the MFI in the <u><b>original scale</b></u> as a function of concentration; (B) Standard curve plotting the MFI in the <u><b>original scale</b></u> as a function of concentration <u><b>after subtracting</b></u> the MFI of the blank control from all standard samples; (C) Standard curve plotting the MFI in the <u><b>log10 scale</b></u> as a function of concentration; and (D) Standard curve plotting the MFI in the <u><b>log10 scale</b></u> as a function of concentration <u><b>after subtracting</b></u> the MFI of the blank control from all standard samples.</p> 2017-11-14 18:41:23 quality control Suboptimal calibration fitness RTS method malaria vaccine multiplex bead-based immunoassays data analysis Multiplex bead-based immunoassays background noise multiplex bead-based immunoassay estimation function SC Phase 3 randomized sample quantification analyte concentration drLumi conduct quality control