M. Tauber, John Brown, Elizabeth B. Li, Yuanyuan Yurgel, Maria E. Masek, Pavel C. Keene, Alex IR56d anterior projections are dispensable for PER to FAs. <p>(A) IR56d and Gr64f neurons visualized with IR56d-GAL4 driving RFP and Gr64f-LexA driving GFP. Co-localization is detected in the posterior projections, but is minimal in anterior projections. (B) Driving the GAL4 repressor Gal80 with Gr64f-LexA limits GFP expression to the non-overlapping IR56d anterior projections. (C) Restricting TNT expression to the non-overlapping anterior projection neurons does not significantly impact PER to sugar or HxA (impTNT n = 23; TNT n = 22). Error bars indicate SEM. Wilcoxon Rank Sum Test Sucrose: <i>p</i>>0.98; HxA: <i>p</i>>0.96. (D) Restricting TRPA1 expression to the non-overlapping neurons abolishes PER compared to control in which TRPA1 is driven in all IR56d neurons. Fisher’s Exact Test with Bonferroni correction for multiple comparisons; ***<i>p</i><0.001.</p> Silencing IR 56d neurons;IR 56d neurons;aversive taste memory assay;sugar-sensing taste neurons;medium-chain FAs;abolishes FA taste;Gr 64f;Gustatory Receptor 64 f;Drosophila gustatory center;acid taste Fat;IR 56d;Ionotropic Receptor 56;FA taste;activates reward circuitry 2017-11-09
    https://plos.figshare.com/articles/figure/IR56d_anterior_projections_are_dispensable_for_PER_to_FAs_/5588047
10.1371/journal.pgen.1007059.g005