TY - DATA T1 - Characterization of a novel alkaline esterase from Altererythrobacter epoxidivorans CGMCC 1.7731T PY - 2017/11/03 AU - Zhen Rong AU - Ying-Yi Huo AU - Shu-Ling Jian AU - Yue-Hong Wu AU - Xue-Wei Xu UR - https://tandf.figshare.com/articles/journal_contribution/Characterization_of_a_novel_alkaline_esterase_from_i_Altererythrobacter_epoxidivorans_i_CGMCC_1_7731_sup_T_sup_/5568706 DO - 10.6084/m9.figshare.5568706.v1 L4 - https://ndownloader.figshare.com/files/9676498 KW - Affinity chromatography purification KW - alkaline esterase KW - Altererythrobacter epoxidivorans CGMCC 1.7731T KW - characterization KW - expression KW - isolation N2 - A novel esterase gene (e25) was identified from Altererythrobacter epoxidivorans CGMCC 1.7731T by genome sequence screening. The e25 gene is 948 nucleotides in length and encodes a 315 amino acid protein (E25) with a predicted molecular mass of 33,683 Da. A phylogenetic tree revealed that E25 belongs to the hormone-sensitive lipase (HSL) family of lipolytic enzymes. An activity assay of E25 showed that it exhibited the highest catalytic efficiency when using p-nitrophenyl caproate (C6) as a substrate. The optimum pH and temperature were determined to be approximately pH 9 and 45°C, and the Km and Vmax values were 0.12 mM and 1,772 µmol/min/mg, respectively. After an incubation at 40°C for 80 min, E25 retained 75% of its basal activity. The enzyme exhibited good tolerance to metal cations, such as Ba2+, Ca2+, and Cu2+ (10 mM), but its activity was strongly inhibited by Co2+, Ni2+, Mn2+, and Zn2+. The E25 enzyme was stimulated by glycerol and retained over 60% of its basal activity in the presence of 1% Tween-80 and Triton X-100. Overall, the activity of E25 under alkaline conditions and its organic solvent and detergent tolerance indicate that E25 could be useful as a novel industrial catalyst in biotechnological applications. ER -