Alcon-Giner, Cristina Caim, Shabhonam Mitra, Suparna Ketskemety, Jennifer Wegmann, Udo Wain, John Belteki, Gusztav Clarke, Paul Hall, Lindsay Additional file 13: Figure S6. of Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants Primer alignment study of the most common bacterial taxa found in ELBW (P29F). a Representation of primers used in this study along the 16S bacterial rRNA gene. b Primer alignment study using 16S rRNA gene from Bifidobacterium bifidum CP 010412 (isolated from Infloran) and the most common bacterial taxa found in an ELBW infant (P29F) with supplementation (Staphylococcus epidermis NR_074995, Enterobacter cloacae CP012165 and Enterococcus faecalis CP014949). We also included two strains of Bifidobacterium as control samples (B.infantis M58738.1 and B.longum ATCC 156697) All sequences are represented in 5′-3’orientation using UPAC nucleotide code, where Y = C or T, R = A or G, K = G or T, M = A or C (PDF 299 kb) Extremely low birth weight infants;Microbiota;16S rRNA gene sequencing;Shotgun sequencing;DNA extraction;Bifidobacterium 2017-11-02
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10.6084/m9.figshare.c.3921094_D4.v1