TY - DATA T1 - Hydrolysis of rhodamine-conjugated Ub-like substrates by chUSP1FL /chUAF1. PY - 2017/11/01 AU - Hainan Zheng AU - Mengyun Wang AU - Chengcheng Zhao AU - Shanli Wu AU - Peifeng Yu AU - Yan Lü AU - Tiedong Wang AU - Yongxing Ai UR - https://plos.figshare.com/articles/figure/Hydrolysis_of_rhodamine-conjugated_Ub-like_substrates_by_chUSP1_sup_FL_sup_chUAF1_/5560093 DO - 10.1371/journal.pone.0186535.g008 L4 - https://ndownloader.figshare.com/files/9656425 KW - chUSP 1 deubiquitination activity KW - chUAF 1 KW - DUB ubiquitin-specific protease 1 KW - 91A KW - USP-associated factor 1 KW - chUSP 1 KW - NEDD KW - substrate specificity KW - MDV KW - DNA damage repair KW - substrate preferences KW - H 594A chUSP 1 mutants KW - SUMO 3-dimers ISG 15-Rho FAT 10-Rho KW - deubiquitination activity KW - 758A KW - chUSP 1 mutants C 91S H 603A KW - SUMO 1-GST SUMO 2- KW - chicken USP 1 homologue N2 - Fluorescence intensity of rhodamine released on the hydrolysis of the rhodamine-conjugated substrates FAT10 (A), ISG15 (B), NEDD8 (C), or Ufm1 (D). The fluorescence intensity traces of either chUSP1FL/chUAF1-containing reactions or negative controls containing an equal volume of buffer (Control) are shown for each substrate. ER -