10.1371/journal.pone.0186535.g008 Hainan Zheng Hainan Zheng Mengyun Wang Mengyun Wang Chengcheng Zhao Chengcheng Zhao Shanli Wu Shanli Wu Peifeng Yu Peifeng Yu Yan Lü Yan Tiedong Wang Tiedong Wang Yongxing Ai Yongxing Ai Hydrolysis of rhodamine-conjugated Ub-like substrates by chUSP1<sup>FL</sup> /chUAF1. Public Library of Science 2017 chUSP 1 deubiquitination activity chUAF 1 DUB ubiquitin-specific protease 1 91A USP-associated factor 1 chUSP 1 NEDD substrate specificity MDV DNA damage repair substrate preferences H 594A chUSP 1 mutants SUMO 3-dimers ISG 15-Rho FAT 10-Rho deubiquitination activity 758A chUSP 1 mutants C 91S H 603A SUMO 1-GST SUMO 2- chicken USP 1 homologue 2017-11-01 17:32:45 Figure https://plos.figshare.com/articles/figure/Hydrolysis_of_rhodamine-conjugated_Ub-like_substrates_by_chUSP1_sup_FL_sup_chUAF1_/5560093 <p>Fluorescence intensity of rhodamine released on the hydrolysis of the rhodamine-conjugated substrates FAT10 (A), ISG15 (B), NEDD8 (C), or Ufm1 (D). The fluorescence intensity traces of either chUSP1<sup>FL</sup>/chUAF1-containing reactions or negative controls containing an equal volume of buffer (Control) are shown for each substrate.</p>