10.1371/journal.pone.0186535.g008
Hainan Zheng
Hainan
Zheng
Mengyun Wang
Mengyun
Wang
Chengcheng Zhao
Chengcheng
Zhao
Shanli Wu
Shanli
Wu
Peifeng Yu
Peifeng
Yu
Yan Lü
Yan
Lü
Tiedong Wang
Tiedong
Wang
Yongxing Ai
Yongxing
Ai
Hydrolysis of rhodamine-conjugated Ub-like substrates by chUSP1<sup>FL</sup> /chUAF1.
Public Library of Science
2017
chUSP 1 deubiquitination activity
chUAF 1
DUB ubiquitin-specific protease 1
91A
USP-associated factor 1
chUSP 1
NEDD
substrate specificity
MDV
DNA damage repair
substrate preferences
H 594A chUSP 1 mutants
SUMO 3-dimers ISG 15-Rho FAT 10-Rho
deubiquitination activity
758A
chUSP 1 mutants C 91S H 603A
SUMO 1-GST SUMO 2-
chicken USP 1 homologue
2017-11-01 17:32:45
Figure
https://plos.figshare.com/articles/figure/Hydrolysis_of_rhodamine-conjugated_Ub-like_substrates_by_chUSP1_sup_FL_sup_chUAF1_/5560093
<p>Fluorescence intensity of rhodamine released on the hydrolysis of the rhodamine-conjugated substrates FAT10 (A), ISG15 (B), NEDD8 (C), or Ufm1 (D). The fluorescence intensity traces of either chUSP1<sup>FL</sup>/chUAF1-containing reactions or negative controls containing an equal volume of buffer (Control) are shown for each substrate.</p>