Niarchos, Athanasios Siora, Anastasia Konstantinou, Evangelia Kalampoki, Vasiliki Lagoumintzis, George Poulas, Konstantinos Western blot analysis of the expressed BRP, CAT and CSFVRP proteins. <p>BL21 <i>E</i>. <i>coli</i> transformed with recombinant pET-BccI/BRP or pET-BccI/CAT were lysed after protein expression and analyzed by Western blot. <b>1</b>, <b>2</b>, <b>3:</b> Lysates from cultures inoculated with colonies transformed with pET-BccI/BRP <b>4, 5, 6:</b> lysates from cultures inoculated with colonies transformed with pET-BccI/CAT and <b>7,8,9:</b> lysates from cultures inoculated with colonies transformed with pET-BccI/ CSFVRP.</p> novel protein-expression vector pET-BccI;PCR;T 4 DNA polymerase;method;protein-coding genes;expression vectors;TA-GC;novel vector pET-BccI;protein expression;restriction endonuclease BccI 2017-11-01
    https://plos.figshare.com/articles/figure/Western_blot_analysis_of_the_expressed_BRP_CAT_and_CSFVRP_proteins_/5560090
10.1371/journal.pone.0186568.g006