10.1371/journal.pone.0187346
Dayanne Silva Monteiro de Almeida
Dayanne Silva Monteiro
de Almeida
Daniel Oliveira Jordão do Amaral
Daniel Oliveira Jordão do
Amaral
Luiz-Eduardo Del-Bem
Luiz-Eduardo
Del-Bem
Emily Bronze dos Santos
Emily Bronze dos
Santos
Raner José Santana Silva
Raner José Santana
Silva
Karina Peres Gramacho
Karina
Peres Gramacho
Michel Vincentz
Michel
Vincentz
Fabienne Micheli
Fabienne
Micheli
Genome-wide identification and characterization of cacao WRKY transcription factors and analysis of their expression in response to witches' broom disease
Public Library of Science
2017
7 TcWRKY genes
fungus Moniliophthora perniciosa
zinc-finger motif types
61 non-redundant WRKY sequences
10 cacao chromosomes
phylogenetic analysis
cacao WRKY transcription factors
Theobroma cacao WRKY TF family
broom disease Transcriptional regulation
III
response
expression
2017-10-30 17:43:28
Dataset
https://plos.figshare.com/articles/dataset/Genome-wide_identification_and_characterization_of_cacao_WRKY_transcription_factors_and_analysis_of_their_expression_in_response_to_witches_broom_disease/5552029
<div><p>Transcriptional regulation, led by transcription factors (TFs) such as those of the WRKY family, is a mechanism used by the organism to enhance or repress gene expression in response to stimuli. Here, we report on the genome-wide analysis of the <i>Theobroma cacao</i> WRKY TF family and also investigate the expression of WRKY genes in cacao infected by the fungus <i>Moniliophthora perniciosa</i>. In the cacao genome, 61 non-redundant WRKY sequences were found and classified in three groups (I to III) according to the WRKY and zinc-finger motif types. The 61 putative WRKY sequences were distributed on the 10 cacao chromosomes and 24 of them came from duplication events. The sequences were phylogenetically organized according to the general WRKY groups. The phylogenetic analysis revealed that subgroups IIa and IIb are sister groups and share a common ancestor, as well as subgroups IId and IIe. The most divergent groups according to the plant origin were IIc and III. According to the phylogenetic analysis, 7 <i>TcWRKY</i> genes were selected and analyzed by RT-qPCR in susceptible and resistant cacao plants infected (or not) with <i>M</i>. <i>perniciosa</i>. Some <i>TcWRKY</i> genes presented interesting responses to <i>M</i>. <i>perniciosa</i> such as Tc01_p014750/Tc06_p013130/AtWRKY28, Tc09_p001530/Tc06_p004420/AtWRKY40, Tc04_p016130/AtWRKY54 and Tc10_p016570/ AtWRKY70. Our results can help to select appropriate candidate genes for further characterization in cacao or in other <i>Theobroma</i> species.</p></div>